Vesicle transport and growth dynamics in Aspergillus niger : Microscale modeling of secretory vesicle flow and centerline extraction from confocal fluorescent data
© 2020 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC..
In this paper, we present a mathematical model to describe filamentous fungal growth based on intracellular secretory vesicles (SVs), which transport cell wall components to the hyphal tip. Vesicular transport inside elongating hyphae is modeled as an advection-diffusion-reaction equation with a moving boundary, transformed into fixed coordinates, and discretized using a high-order weighted essentially nonoscillatory discretization scheme. The model describes the production and the consumption of SVs with kinetic functions. Simulations are subsequently compared against distributions of SVs visualized by enhanced green fluorescent protein in young Aspergillus niger hyphae after germination. Intensity profile data are obtained using an algorithm scripted in ImageJ that extracts mean intensity distributions from 3D time-lapse confocal measurement data. Simulated length growth is in good agreement with the experimental data. Our simulations further show that a decrease of effective vesicle transport velocity towards the tip can explain the observed tip accumulation of SVs.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:117 |
|---|---|
| Enthalten in: |
Biotechnology and bioengineering - 117(2020), 9 vom: 01. Sept., Seite 2875-2886 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Kunz, Philipp J [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 13.09.2021 Date Revised 13.09.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/bit.27452 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM310896606 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM310896606 | ||
| 003 | DE-627 | ||
| 005 | 20231225141102.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/bit.27452 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1036.xml |
| 035 | |a (DE-627)NLM310896606 | ||
| 035 | |a (NLM)32510171 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kunz, Philipp J |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Vesicle transport and growth dynamics in Aspergillus niger |b Microscale modeling of secretory vesicle flow and centerline extraction from confocal fluorescent data |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 13.09.2021 | ||
| 500 | |a Date Revised 13.09.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC. | ||
| 520 | |a In this paper, we present a mathematical model to describe filamentous fungal growth based on intracellular secretory vesicles (SVs), which transport cell wall components to the hyphal tip. Vesicular transport inside elongating hyphae is modeled as an advection-diffusion-reaction equation with a moving boundary, transformed into fixed coordinates, and discretized using a high-order weighted essentially nonoscillatory discretization scheme. The model describes the production and the consumption of SVs with kinetic functions. Simulations are subsequently compared against distributions of SVs visualized by enhanced green fluorescent protein in young Aspergillus niger hyphae after germination. Intensity profile data are obtained using an algorithm scripted in ImageJ that extracts mean intensity distributions from 3D time-lapse confocal measurement data. Simulated length growth is in good agreement with the experimental data. Our simulations further show that a decrease of effective vesicle transport velocity towards the tip can explain the observed tip accumulation of SVs | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Aspergillus niger | |
| 650 | 4 | |a filamentous fungi | |
| 650 | 4 | |a germ tube | |
| 650 | 4 | |a modeling | |
| 650 | 4 | |a protein secretion | |
| 650 | 4 | |a secretory vesicle | |
| 650 | 7 | |a enhanced green fluorescent protein |2 NLM | |
| 650 | 7 | |a Green Fluorescent Proteins |2 NLM | |
| 650 | 7 | |a 147336-22-9 |2 NLM | |
| 700 | 1 | |a Barthel, Lars |e verfasserin |4 aut | |
| 700 | 1 | |a Meyer, Vera |e verfasserin |4 aut | |
| 700 | 1 | |a King, Rudibert |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biotechnology and bioengineering |d 1976 |g 117(2020), 9 vom: 01. Sept., Seite 2875-2886 |w (DE-627)NLM000014702 |x 1097-0290 |7 nnns |
| 773 | 1 | 8 | |g volume:117 |g year:2020 |g number:9 |g day:01 |g month:09 |g pages:2875-2886 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/bit.27452 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 117 |j 2020 |e 9 |b 01 |c 09 |h 2875-2886 | ||