Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex
© 2020 Wiley Periodicals LLC..
The Hippo pathway has been associated with regulation of early follicle growth. Studies of murine ovaries suggest that changes in the actin cytoskeleton, caused by fragmentation, result in inhibition of the Hippo pathway, and in turn, may activate follicle growth. In humans, the connections between fragmentation, the actin cytoskeleton, and follicle activation are yet to be confirmed. In this study, we investigated the impact in vitro fragmentation of a human ovarian cortex on (a) actin polymerization, (b) components of the Hippo pathway, and (c) follicle growth in vivo. The results showed that the ratio between globular and filamentous actin remained unchanged at all timepoints (0, 10, 30, 60, 120, and 240 min) following tissue fragmentation. Neither was the Hippo pathway effector protein YES-associated protein upregulated nor was gene expression of the downstream growth factors CCN2, CCN3, or CCN5 increased at any timepoint in the fragmented cortex. Furthermore, the number of growing follicles was similar in fragmented and intact cortex pieces after 6 weeks' xenotransplantation. However, the total number of surviving follicles was considerably lower in the fragmented cortex compared with intact tissue, suggesting detrimental effects of fragmentation on tissue grafting. These results indicate that fragmentation is likely to be ineffective to activate follicle growth in the human ovarian cortex.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:87 |
|---|---|
| Enthalten in: |
Molecular reproduction and development - 87(2020), 6 vom: 17. Juni, Seite 711-719 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Lunding, Stine A [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Actin |
|---|
| Anmerkungen: |
Date Completed 22.07.2021 Date Revised 04.12.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/mrd.23353 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM310863082 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM310863082 | ||
| 003 | DE-627 | ||
| 005 | 20231225141020.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/mrd.23353 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1036.xml |
| 035 | |a (DE-627)NLM310863082 | ||
| 035 | |a (NLM)32506789 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Lunding, Stine A |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.07.2021 | ||
| 500 | |a Date Revised 04.12.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 Wiley Periodicals LLC. | ||
| 520 | |a The Hippo pathway has been associated with regulation of early follicle growth. Studies of murine ovaries suggest that changes in the actin cytoskeleton, caused by fragmentation, result in inhibition of the Hippo pathway, and in turn, may activate follicle growth. In humans, the connections between fragmentation, the actin cytoskeleton, and follicle activation are yet to be confirmed. In this study, we investigated the impact in vitro fragmentation of a human ovarian cortex on (a) actin polymerization, (b) components of the Hippo pathway, and (c) follicle growth in vivo. The results showed that the ratio between globular and filamentous actin remained unchanged at all timepoints (0, 10, 30, 60, 120, and 240 min) following tissue fragmentation. Neither was the Hippo pathway effector protein YES-associated protein upregulated nor was gene expression of the downstream growth factors CCN2, CCN3, or CCN5 increased at any timepoint in the fragmented cortex. Furthermore, the number of growing follicles was similar in fragmented and intact cortex pieces after 6 weeks' xenotransplantation. However, the total number of surviving follicles was considerably lower in the fragmented cortex compared with intact tissue, suggesting detrimental effects of fragmentation on tissue grafting. These results indicate that fragmentation is likely to be ineffective to activate follicle growth in the human ovarian cortex | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Hippo pathway | |
| 650 | 4 | |a actin | |
| 650 | 4 | |a female infertility | |
| 650 | 4 | |a follicle activation | |
| 650 | 4 | |a ovarian cortex | |
| 650 | 7 | |a Actins |2 NLM | |
| 650 | 7 | |a Protein Serine-Threonine Kinases |2 NLM | |
| 650 | 7 | |a EC 2.7.11.1 |2 NLM | |
| 700 | 1 | |a Andersen, Anders N |e verfasserin |4 aut | |
| 700 | 1 | |a Hardardottir, Lilja |e verfasserin |4 aut | |
| 700 | 1 | |a Olesen, Hanna Ø |e verfasserin |4 aut | |
| 700 | 1 | |a Kristensen, Stine G |e verfasserin |4 aut | |
| 700 | 1 | |a Andersen, Claus Y |e verfasserin |4 aut | |
| 700 | 1 | |a Pors, Susanne E |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular reproduction and development |d 1994 |g 87(2020), 6 vom: 17. Juni, Seite 711-719 |w (DE-627)NLM012606154 |x 1098-2795 |7 nnns |
| 773 | 1 | 8 | |g volume:87 |g year:2020 |g number:6 |g day:17 |g month:06 |g pages:711-719 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/mrd.23353 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 87 |j 2020 |e 6 |b 17 |c 06 |h 711-719 | ||