Cardiomyocyte Proteome Remodeling due to Isoproterenol-Induced Cardiac Hypertrophy during the Compensated Phase

© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim..

PURPOSE: Although the pathophysiological response of cardiac tissue to pro-hypertrophic stimulus is well characterized, a comprehensive characterization of the molecular events underlying the pathological hypertrophy in cardiomyocytes during the early compensated cardiac hypertrophy is currently lacking.

EXPERIMENTAL DESIGN: A quantitative label-free proteomic analysis of cardiomyocytes isolated was conducted from mice treated subcutaneously with isoproterenol (ISO) during 7 days in comparison with cardiomyocytes from control animals (CT).

RESULTS: Canonical pathway analysis of dysregulated proteins indicated that ISO-hypertrophy drives the activation of actin cytoskeleton and integrin-linked kinase (ILK) signaling, and inhibition of the sirtuin signaling. Alteration in cardiac contractile function and calcium signaling are predicted as downstream effects of ISO-hypertrophy probably due to the upregulation of key elements such as myosin-7 (MYH7). Confocal microscopy corroborated that indeed ISO-treatment led to increased abundance of MYH7. Potential early markers for cardiac hypertrophy as APBB1, GOLGA4, HOOK1, KATNA1, KIFBP, MAN2B2, and SLC16A1 are also reported.

CONCLUSIONS AND CLINICAL RELEVANCE: The data consist in a complete molecular mapping of ISO-induced compensated cardiac hypertrophy model at cardiomyocyte level. Marker candidates reported may assist early diagnosis of cardiac hypertrophy and ultimately heart failure.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:14

Enthalten in:

Proteomics. Clinical applications - 14(2020), 4 vom: 17. Juli, Seite e2000017

Sprache:

Englisch

Beteiligte Personen:

Parreira, Ricardo Cambraia [VerfasserIn]
Gómez-Mendoza, Diana Paola [VerfasserIn]
de Jesus, Itamar Couto Guedes [VerfasserIn]
Lemos, Rafael Pereira [VerfasserIn]
Santos, Anderson Kennedy [VerfasserIn]
Rezende, Cristiana Perdigão [VerfasserIn]
Figueiredo, Henrique César Pereira [VerfasserIn]
Pinto, Mauro Cunha Xavier [VerfasserIn]
Kjeldsen, Frank [VerfasserIn]
Guatimosim, Silvia [VerfasserIn]
Resende, Rodrigo Ribeiro [VerfasserIn]
Verano-Braga, Thiago [VerfasserIn]

Links:

Volltext

Themen:

Actins
Cardiac hypertrophy
Cardiomyocyte
EC 2.7.1.-
EC 2.7.11.1
EC 3.6.4.1
Integrin-linked kinase
Isoproterenol
Journal Article
L628TT009W
Mass spectrometry
Myh7 protein, mouse
Myosin Heavy Chains
Protein Serine-Threonine Kinases
Proteome
Proteomics
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 03.06.2021

Date Revised 04.12.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1002/prca.202000017

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM310863074