Use of Immunostaining for the diagnosis of Lymphovascular invasion in superficial Barrett's esophageal adenocarcinoma

BACKGROUND: The prevalence of Barrett's esophageal adenocarcinoma (BEA) is increasing in Japan. Accurate assessment of lymphovascular invasion (LVI) after endoscopic resection or surgery is essential in evaluating treatment response. This study aimed to assess the usefulness of immunostaining in determining the extent of LVI in superficial BEA.

METHODS: We retrospectively included 41 patients who underwent endoscopic resection or surgery between January 2007 and July 2018. In all cases, 3-μm serial sections from paraffin-embedded resected specimens were used for hematoxylin and eosin (H-E) staining and immunostaining for D2-40 and CD31. Two specialized gastrointestinal pathologists (T.Y. and T.T.), blinded to clinical information, independently evaluated the extent of LVI from these specimens. The LVI-positivity rate was evaluated with respect to the depth of invasion, changes in the positivity rate on immunostaining, pathological characteristics of patients with LVI, lymph node metastasis or relapse, and course after treatment.

RESULTS: H-E staining alone identified LVI in 7 patients (positivity rate: 17.1%). Depths of invasion were categorized based on extension to the submucosa (SM) or deeper. On immunostaining for D2-40 and CD31, additional positivity was detected in 2 patients with SM1 and 1 SM3, respectively; LVI was detected in 10 patients (positivity rate: 24.4%). LVI-positivity rates with invasion of the superficial muscularis mucosa (SMM)/lamina propria mucosa (LPM)/deep muscularis mucosa (DMM), SM 1, 2, and 3 were 0, 75, 28.6, and 55.6%, respectively.

CONCLUSIONS: Combined H-E staining and immunostaining is useful in diagnosing LVI in superficial BEA, particularly in endoscopically resected specimens.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:20

Enthalten in:

BMC gastroenterology - 20(2020), 1 vom: 05. Juni, Seite 175

Sprache:

Englisch

Beteiligte Personen:

Hosono, Isao [VerfasserIn]
Miyahara, Ryoji [VerfasserIn]
Furukawa, Kazuhiro [VerfasserIn]
Funasaka, Kohei [VerfasserIn]
Sawada, Tsunaki [VerfasserIn]
Maeda, Keiko [VerfasserIn]
Yamamura, Takeshi [VerfasserIn]
Ishikawa, Takuya [VerfasserIn]
Ohno, Eizaburo [VerfasserIn]
Nakamura, Masanao [VerfasserIn]
Kawashima, Hiroki [VerfasserIn]
Yokoi, Takio [VerfasserIn]
Tsukamoto, Tetsuya [VerfasserIn]
Hirooka, Yoshiki [VerfasserIn]
Fujishiro, Mitsuhiro [VerfasserIn]

Links:

Volltext

Themen:

Antibodies, Monoclonal, Murine-Derived
Barrett’s esophageal adenocarcinoma
CD31
D2–40
Eosine Yellowish-(YS)
Evaluation Study
Hematoxylin
Journal Article
Monoclonal antibody D2-40
PECAM1 protein, human
Platelet Endothelial Cell Adhesion Molecule-1
TDQ283MPCW
YKM8PY2Z55

Anmerkungen:

Date Completed 18.03.2021

Date Revised 18.03.2021

published: Electronic

Citation Status MEDLINE

doi:

10.1186/s12876-020-01319-7

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM310829941