Acquired contractile ability in human endometrial stromal cells by passive loading of cyclic tensile stretch

The uterus plays an important and unique role during pregnancy and is a dynamic organ subjected to mechanical stimuli. It has been reported that infertility occurs when the peristalsis is prevented, although its mechanisms remain unknown. In this study, we found that mechanical strain mimicking the peristaltic motion of the uterine smooth muscle layer enabled the endometrial stromal cells to acquire contractility. In order to mimic the peristalsis induced by uterine smooth muscle cells, cyclic tensile stretch was applied to human endometrial stromal cells. The results showed that the strained cells exerted greater contractility in three-dimensional collagen gels in the presence of oxytocin, due to up-regulated alpha-smooth muscle actin expression via the cAMP signaling pathway. These in vitro findings underscore the plasticity of the endometrial stromal cell phenotype and suggest the possibility of acquired contractility by these cells in vivo and its potential contribution to uterine contractile activity. This phenomenon may be a typical example of how a tissue passively acquires new contractile functions under mechanical stimulation from a neighboring tissue, enabling it to support the adjacent tissue's functions.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:10

Enthalten in:

Scientific reports - 10(2020), 1 vom: 02. Juni, Seite 9014

Sprache:

Englisch

Beteiligte Personen:

Kim, Jeonghyun [VerfasserIn]
Ushida, Takashi [VerfasserIn]
Montagne, Kevin [VerfasserIn]
Hirota, Yasushi [VerfasserIn]
Yoshino, Osamu [VerfasserIn]
Hiraoka, Takehiro [VerfasserIn]
Osuga, Yutaka [VerfasserIn]
Furuakwa, Katsuko S [VerfasserIn]

Links:

Volltext

Themen:

50-56-6
56092-81-0
ACTA2 protein, human
Actins
Collagen Type I
Cyclic AMP
E0399OZS9N
Ionomycin
Isoquinolines
Journal Article
M876330O56
N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
Oxytocin
Research Support, Non-U.S. Gov't
Sulfonamides

Anmerkungen:

Date Completed 17.12.2020

Date Revised 02.06.2021

published: Electronic

Citation Status MEDLINE

doi:

10.1038/s41598-020-65884-3

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM310682991