Dose dependent enhanced antibacterial effects and reduced biofilm activity against Bacillus subtilis in presence of ZnO nanoparticles

Copyright © 2020 Elsevier B.V. All rights reserved..

Bacterial biofilms are self-produced matrix of sticky extracellular polymeric substances. They result in fouling in the food industry, water treatment plants, and possess significant environmental and industrial impacts. Nanoparticles have shown immense potential and have been effective in combating bacterial biofilm, which is the common cause of drug resistance development, biofouling in water treatment plants and the food industry. Hence, in order to explore the same, Zinc oxide nanoparticles have been synthesized by chemical synthesis method and their action against Bacillus subtilis biofilm formation was evaluated in this study by crystal violet and ROS assay. The dose-dependent reduction in biofilm biomass and density was observed as a result of nanoparticle exposure. There was considerable reduction in biofilm formation after treatment with ZnO nanoparticles. Change in surface morphology of the Bacillus subtilis cells was observed which could be due to oxidative stress induced by ZnO nanoparticles. The oxidative stress was estimated by measurement of catalase activity that also showed dose-dependent decrease.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:113

Enthalten in:

Materials science & engineering. C, Materials for biological applications - 113(2020) vom: 10. Aug., Seite 111021

Sprache:

Englisch

Beteiligte Personen:

Awasthi, Anjali [VerfasserIn]
Sharma, Puja [VerfasserIn]
Jangir, Lokesh [VerfasserIn]
Kamakshi [VerfasserIn]
Awasthi, Garima [VerfasserIn]
Awasthi, Kumud Kant [VerfasserIn]
Awasthi, Kamlendra [VerfasserIn]

Links:

Volltext

Themen:

Anti-Bacterial Agents
Bacillus subtilis
Biofilm
Catalase activity
Gentian Violet
J4Z741D6O5
Journal Article
Oxidative stress
Reactive Oxygen Species
SOI2LOH54Z
Zinc Oxide
ZnO nanoparticles

Anmerkungen:

Date Completed 25.03.2021

Date Revised 25.03.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.msec.2020.111021

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM31067624X