Combinatorial Methylerythritol Phosphate Pathway Engineering and Process Optimization for Increased Menaquinone-7 Synthesis in Bacillus subtilis
Vitamin K2 (menaquinone) is an essential vitamin existing in the daily diet, and menaquinone-7 (MK-7) is an important form of it. In a recent work, we engineered the synthesis modules of MK-7 in Bacillus subtilis, and the strain BS20 could produce 360 mg/l MK-7 in shake flasks, while the methylerythritol phosphate (MEP) pathway, which provides the precursor isopentenyl diphosphate for MK-7 synthesis, was not engineered. In this study, we overexpressed five genes of the MEP pathway in BS20 and finally obtained a strain (BS20DFHG) with MK-7 titer of 415 mg/l in shake flasks. Next, we optimized the fermentation process parameters (initial pH, temperature and aeration) in an 8-unit parallel bioreactor system consisting of 300-ml glass vessels. Based on this, we scaled up the MK-7 production by the strain BS20DFHG in a 50-l bioreactor, and the highest MK-7 titer reached 242 mg/l. Here, we show that the engineered strain BS20DFHG may be used for the industrial production of MK-7 in the future.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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Enthalten in: |
Journal of microbiology and biotechnology - 30(2020), 5 vom: 28. Mai, Seite 762-769 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Chen, Taichi [VerfasserIn] |
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Links: |
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Themen: |
11032-49-8 |
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Anmerkungen: |
Date Completed 22.01.2021 Date Revised 29.02.2024 published: Print Citation Status MEDLINE |
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doi: |
10.4014/jmb.1912.12008 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM310633125 |
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520 | |a Vitamin K2 (menaquinone) is an essential vitamin existing in the daily diet, and menaquinone-7 (MK-7) is an important form of it. In a recent work, we engineered the synthesis modules of MK-7 in Bacillus subtilis, and the strain BS20 could produce 360 mg/l MK-7 in shake flasks, while the methylerythritol phosphate (MEP) pathway, which provides the precursor isopentenyl diphosphate for MK-7 synthesis, was not engineered. In this study, we overexpressed five genes of the MEP pathway in BS20 and finally obtained a strain (BS20DFHG) with MK-7 titer of 415 mg/l in shake flasks. Next, we optimized the fermentation process parameters (initial pH, temperature and aeration) in an 8-unit parallel bioreactor system consisting of 300-ml glass vessels. Based on this, we scaled up the MK-7 production by the strain BS20DFHG in a 50-l bioreactor, and the highest MK-7 titer reached 242 mg/l. Here, we show that the engineered strain BS20DFHG may be used for the industrial production of MK-7 in the future | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Bacillus subtilis | |
650 | 4 | |a Menaquinone | |
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700 | 1 | |a Xia, Hongzhi |e verfasserin |4 aut | |
700 | 1 | |a Cui, Shixiu |e verfasserin |4 aut | |
700 | 1 | |a Lv, Xueqin |e verfasserin |4 aut | |
700 | 1 | |a Li, Xueliang |e verfasserin |4 aut | |
700 | 1 | |a Liu, Yanfeng |e verfasserin |4 aut | |
700 | 1 | |a Li, Jianghua |e verfasserin |4 aut | |
700 | 1 | |a Du, Guocheng |e verfasserin |4 aut | |
700 | 1 | |a Liu, Long |e verfasserin |4 aut | |
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