Alterations of aorta intima and media transcriptome in swine fed high-fat diet over 1-year follow-up period and of the switch to normal diet

Copyright © 2020 The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved..

BACKGROUND AND AIM: We previously showed that 12-month high-fat diet (HFD) in pigs led to fattening and increased artery intima-media-thickness, which were partly reversed after 3-month return to control diet (CD). The aim of this study was to decipher underlying mechanism of action by using transcriptomic analyses of intima and media of aorta.

METHODS AND RESULTS: Thirty-two pigs were divided into three groups: CD for 12 months; HFD for 12 months; switch diet group (regression diet; RD): HFD for 9 months followed by CD for 3 months. After 12 months, RNA was isolated from aorta intima and media for nutrigenomic analyses. HFD significantly affected gene expression in intima, while RD gene expression profile was distinct from the CD group. This suggests that switch to CD is not sufficient to correct gene expression alterations induced by HFD but counteracted expression of a group of genes. HFD also affected gene expression in media and as for intima, the expression profile of media of pigs on RD differed from that of these on CD.

CONCLUSIONS: This study revealed nutrigenomic modifications induced by long-term HFD consumption on arterial intima and media. The return to CD was not sufficient to counteract the genomic effect of HFD.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:30

Enthalten in:

Nutrition, metabolism, and cardiovascular diseases : NMCD - 30(2020), 7 vom: 25. Juni, Seite 1201-1215

Sprache:

Englisch

Beteiligte Personen:

Milenkovic, D [VerfasserIn]
Paslawski, R [VerfasserIn]
Gomulkiewicz, A [VerfasserIn]
Gladine, C [VerfasserIn]
Janczak, D [VerfasserIn]
Grzegorek, I [VerfasserIn]
Jablonska, K [VerfasserIn]
Drozdz, K [VerfasserIn]
Chmielewska, M [VerfasserIn]
Piotrowska, A [VerfasserIn]
Janiszewski, A [VerfasserIn]
Dziegiel, P [VerfasserIn]
Mazur, A [VerfasserIn]
Paslawska, U [VerfasserIn]
Szuba, A [VerfasserIn]

Links:

Volltext

Themen:

Atherosclerosis
Endothelium
High-fat diet
Journal Article
Obesity
Research Support, Non-U.S. Gov't
Swine
Transcriptomics
Vascular

Anmerkungen:

Date Completed 13.10.2020

Date Revised 13.10.2020

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.numecd.2020.04.007

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM310628504