A mouse model that is immunologically tolerant to reporter and modifier proteins
Reporter proteins have become an indispensable tool in biomedical research. However, exogenous introduction of these reporters into mice poses a risk of rejection by the immune system. Here, we describe the generation, validation and application of a multiple reporter protein tolerant 'Tol' mouse model that constitutively expresses an assembly of shuffled reporter proteins from a single open reading frame. We demonstrate that expression of the Tol transgene results in the deletion of CD8+ T cells specific for a model epitope, and substantially improves engraftment of reporter-gene transduced T cells. The Tol strain provides a valuable mouse model for cell transfer and viral-mediated gene transfer studies, and serves as a methodological example for the generation of poly-tolerant mouse strains.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:3 |
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| Enthalten in: |
Communications biology - 3(2020), 1 vom: 29. Mai, Seite 273 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bresser, Kaspar [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Completed 14.06.2021 Date Revised 14.06.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s42003-020-0979-0 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM310524857 |
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| 245 | 1 | 2 | |a A mouse model that is immunologically tolerant to reporter and modifier proteins |
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| 500 | |a Date Revised 14.06.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Reporter proteins have become an indispensable tool in biomedical research. However, exogenous introduction of these reporters into mice poses a risk of rejection by the immune system. Here, we describe the generation, validation and application of a multiple reporter protein tolerant 'Tol' mouse model that constitutively expresses an assembly of shuffled reporter proteins from a single open reading frame. We demonstrate that expression of the Tol transgene results in the deletion of CD8+ T cells specific for a model epitope, and substantially improves engraftment of reporter-gene transduced T cells. The Tol strain provides a valuable mouse model for cell transfer and viral-mediated gene transfer studies, and serves as a methodological example for the generation of poly-tolerant mouse strains | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 700 | 1 | |a Dijkgraaf, Feline E |e verfasserin |4 aut | |
| 700 | 1 | |a Pritchard, Colin E J |e verfasserin |4 aut | |
| 700 | 1 | |a Huijbers, Ivo J |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Ji-Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Rohr, Jan C |e verfasserin |4 aut | |
| 700 | 1 | |a Scheeren, Ferenc A |e verfasserin |4 aut | |
| 700 | 1 | |a Schumacher, Ton N |e verfasserin |4 aut | |
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