Genetic Regulation of Redox Balance in β-Thalassemia Trait

β-Thalassemia (β-thal) trait is a heterogeneous group of genetic defects leading to decreased β-globin production, ineffective erythropoiesis, and oxidative stress. The aim is to evaluate the cytoprotective response, at transcriptional and systemic levels, of the variations of global redox balance in β-thal trait patients. Sixty-six subjects (40 healthy and 26 with β-thal trait) were analyzed at the Universidad Nacional de Tucumán, Tucumán, Argentina, between 2016 and 2017. The following parameters were evaluated: complete blood count, iron status, hemoglobin (Hb) electrophoresis, Hb A2, thiobarbituric acid reactive species (TBARS), serum catalase (CAT), and superoxide dismutase (SOD) activity, FOXO3a, NRF2, SOD, PRDX2, CAT, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) gene expression. The β-thal trait group showed a decrease in Hb levels, MCV, and MCH with higher TBARS levels. The SOD activity was significantly increased by 32.0% in β-thal trait patients respect to the control group. Relative expression of NRF2 was 4.7-fold higher in β-thal trait than in the control group, while FOXO3a expression was similar in both groups. The SOD, PRDX2, and proinflammatory cytokines transcriptional expression was significantly upregulated in β-thal trait patients. This is the first study on the genetic regulation of redox balance in β-thal trait patients in which interesting modifications were observed in the transcript levels of some redox regulators that could be associated with changes in the erythrocyte proteome in this disorder. A better understanding of the pathophysiological mechanisms present in these heterozygous patients would allow adequate therapy in situations such as growth, pregnancy, or high performance sports, favoring a personalized treatment.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:44

Enthalten in:

Hemoglobin - 44(2020), 2 vom: 02. März, Seite 122-127

Sprache:

Englisch

Beteiligte Personen:

Terán, Magdalena M [VerfasserIn]
Mónaco, Maria E [VerfasserIn]
Lazarte, Sandra S [VerfasserIn]
Haro, Cecilia [VerfasserIn]
Ledesma Achem, Emilse [VerfasserIn]
Asensio, Natalia A [VerfasserIn]
Issé, Blanca A [VerfasserIn]

Links:

Volltext

Themen:

β-Thalassemia (β-thal) trait
Beta-Globins
Journal Article
NRF2
Oxidative stress
Proinflammatory cytokines

Anmerkungen:

Date Completed 09.04.2021

Date Revised 09.04.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1080/03630269.2020.1765794

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM310301874