Dust mite-derived Enterobacterial fimbriae H protein enforces the allergen specific immunotherapy in asthma mice
Copyright © 2020 SEICAP. Published by Elsevier España, S.L.U. All rights reserved..
BACKGROUND: The mite alimentary canal contains plenty of microbiota. It is accepted that some of the microbial products function as adjuvants to speed up immune responses.
OBJECTIVES: We identified five bacterial proteins from dust mite, and Enterobacterial fimbriae H (FimH) was one of them. This study aims to test a hypothesis that the FimH protein enforces immunotherapy in asthmatic mice.
METHODS: Asthmatic mice were treated by allergen specific immunotherapy (ASIT) with rDer f1/f2 or rDer f1/f2 plus FimH. Changes in inflammatory cell infiltration, airway hyperreactivity, frequency of Tregs, splenic CD4+IFN-γ+ cells, and serum levels of TGF-β, IL-10, IL-13 and IL-17A of asthmatic mice were checked.
RESULTS: ASIT with rDer f1/f2 plus FimH reduced inflammatory cell infiltration, airway hyperreactivity (AHR), and levels of IgE and IgG1 compared to ASIT with rDer f1/f2 alone, but the levels of IgG2a increased. Asthmatic mice that underwent ASIT with rDer f1/f2 plus FimH showed increased frequency of Tregs, splenic CD4+IFN-γ+ cells, serum levels of TGF-β and IL-10; and deceased splenic CD4+IL-4+ cells, and serum levels of IL-13 and IL-17A. In vitro study showed FimH triggered IL-10 expression in a concentration dependent manner and facilitated the differentiation of Tregs.
CONCLUSION: Used as an adjuvant, FimH enforces the effect of ASIT in asthmatic mice via augmenting Tregs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
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| Enthalten in: |
Allergologia et immunopathologia - 48(2020), 6 vom: 01. Nov., Seite 654-665 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yang, X [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 06.09.2021 Date Revised 06.09.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.aller.2020.03.012 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM310289629 |
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| 100 | 1 | |a Yang, X |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Dust mite-derived Enterobacterial fimbriae H protein enforces the allergen specific immunotherapy in asthma mice |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Completed 06.09.2021 | ||
| 500 | |a Date Revised 06.09.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 SEICAP. Published by Elsevier España, S.L.U. All rights reserved. | ||
| 520 | |a BACKGROUND: The mite alimentary canal contains plenty of microbiota. It is accepted that some of the microbial products function as adjuvants to speed up immune responses | ||
| 520 | |a OBJECTIVES: We identified five bacterial proteins from dust mite, and Enterobacterial fimbriae H (FimH) was one of them. This study aims to test a hypothesis that the FimH protein enforces immunotherapy in asthmatic mice | ||
| 520 | |a METHODS: Asthmatic mice were treated by allergen specific immunotherapy (ASIT) with rDer f1/f2 or rDer f1/f2 plus FimH. Changes in inflammatory cell infiltration, airway hyperreactivity, frequency of Tregs, splenic CD4+IFN-γ+ cells, and serum levels of TGF-β, IL-10, IL-13 and IL-17A of asthmatic mice were checked | ||
| 520 | |a RESULTS: ASIT with rDer f1/f2 plus FimH reduced inflammatory cell infiltration, airway hyperreactivity (AHR), and levels of IgE and IgG1 compared to ASIT with rDer f1/f2 alone, but the levels of IgG2a increased. Asthmatic mice that underwent ASIT with rDer f1/f2 plus FimH showed increased frequency of Tregs, splenic CD4+IFN-γ+ cells, serum levels of TGF-β and IL-10; and deceased splenic CD4+IL-4+ cells, and serum levels of IL-13 and IL-17A. In vitro study showed FimH triggered IL-10 expression in a concentration dependent manner and facilitated the differentiation of Tregs | ||
| 520 | |a CONCLUSION: Used as an adjuvant, FimH enforces the effect of ASIT in asthmatic mice via augmenting Tregs | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Adjuvant effect. | |
| 650 | 4 | |a Allergen specific immunotherapy | |
| 650 | 4 | |a Enterobacterial fimbriae H protein | |
| 650 | 4 | |a House dust mite | |
| 650 | 4 | |a Tregs | |
| 650 | 7 | |a Adjuvants, Immunologic |2 NLM | |
| 650 | 7 | |a Allergens |2 NLM | |
| 650 | 7 | |a Antigens, Bacterial |2 NLM | |
| 650 | 7 | |a Antigens, Dermatophagoides |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a DNA-Binding Proteins |2 NLM | |
| 650 | 7 | |a Recombinant Proteins |2 NLM | |
| 650 | 7 | |a Vaccines, Synthetic |2 NLM | |
| 650 | 7 | |a histone-like protein HU, bacteria |2 NLM | |
| 700 | 1 | |a Wang, H |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, D |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, J |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, X |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, X |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, M |e verfasserin |4 aut | |
| 700 | 1 | |a Li, G |e verfasserin |4 aut | |
| 700 | 1 | |a Ran, P |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, P |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Z |e verfasserin |4 aut | |
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