Exploratory Analysis of CA125-MGL and -STn Glycoforms in the Differential Diagnostics of Pelvic Masses
© American Association for Clinical Chemistry 2020. All rights reserved. For permissions, please email: journals.permissionsoup.com..
BACKGROUND: The cancer antigen 125 (CA125) immunoassay (IA) does not distinguish epithelial ovarian cancer (EOC) from benign disease with the sensitivity needed in clinical practice. In recent studies, glycoforms of CA125 have shown potential as biomarkers in EOC. Here, we assessed the diagnostic abilities of two recently developed CA125 glycoform assays for patients with a pelvic mass. Detailed analysis was further conducted for postmenopausal patients with marginally elevated conventionally measured CA125 levels, as this subgroup presents a diagnostic challenge in the clinical setting.
METHODS: Our study population contained 549 patients diagnosed with EOC, benign ovarian tumors, and endometriosis. Of these, 288 patients were postmenopausal, and 98 of them presented with marginally elevated serum levels of conventionally measured CA125 at diagnosis. Preoperative serum levels of conventionally measured CA125 and its glycoforms (CA125-MGL and CA125-STn) were determined.
RESULTS: The CA125-STn assay identified EOC significantly better than the conventional CA125-IA in postmenopausal patients (85% vs. 74% sensitivity at a fixed specificity of 90%, P = 0.0009). Further, both glycoform assays had superior AUCs compared to the conventional CA125-IA in postmenopausal patients with marginally elevated CA125. Importantly, the glycoform assays reduced the false positive rate of the conventional CA125-IA.
CONCLUSIONS: The results indicate that the CA125 glycoform assays markedly improve the performance of the conventional CA125-IA in the differential diagnosis of pelvic masses. This result is especially valuable when CA125 is marginally elevated.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:5 |
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| Enthalten in: |
The journal of applied laboratory medicine - 5(2020), 2 vom: 01. März, Seite 263-272 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Salminen, Liina [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 09.06.2021 Date Revised 09.06.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/jalm/jfz012 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM310275806 |
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| 100 | 1 | |a Salminen, Liina |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Exploratory Analysis of CA125-MGL and -STn Glycoforms in the Differential Diagnostics of Pelvic Masses |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Completed 09.06.2021 | ||
| 500 | |a Date Revised 09.06.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © American Association for Clinical Chemistry 2020. All rights reserved. For permissions, please email: journals.permissionsoup.com. | ||
| 520 | |a BACKGROUND: The cancer antigen 125 (CA125) immunoassay (IA) does not distinguish epithelial ovarian cancer (EOC) from benign disease with the sensitivity needed in clinical practice. In recent studies, glycoforms of CA125 have shown potential as biomarkers in EOC. Here, we assessed the diagnostic abilities of two recently developed CA125 glycoform assays for patients with a pelvic mass. Detailed analysis was further conducted for postmenopausal patients with marginally elevated conventionally measured CA125 levels, as this subgroup presents a diagnostic challenge in the clinical setting | ||
| 520 | |a METHODS: Our study population contained 549 patients diagnosed with EOC, benign ovarian tumors, and endometriosis. Of these, 288 patients were postmenopausal, and 98 of them presented with marginally elevated serum levels of conventionally measured CA125 at diagnosis. Preoperative serum levels of conventionally measured CA125 and its glycoforms (CA125-MGL and CA125-STn) were determined | ||
| 520 | |a RESULTS: The CA125-STn assay identified EOC significantly better than the conventional CA125-IA in postmenopausal patients (85% vs. 74% sensitivity at a fixed specificity of 90%, P = 0.0009). Further, both glycoform assays had superior AUCs compared to the conventional CA125-IA in postmenopausal patients with marginally elevated CA125. Importantly, the glycoform assays reduced the false positive rate of the conventional CA125-IA | ||
| 520 | |a CONCLUSIONS: The results indicate that the CA125 glycoform assays markedly improve the performance of the conventional CA125-IA in the differential diagnosis of pelvic masses. This result is especially valuable when CA125 is marginally elevated | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CA125 | |
| 650 | 4 | |a differential diagnostics | |
| 650 | 4 | |a epithelial ovarian cancer | |
| 650 | 4 | |a glycosylation | |
| 650 | 4 | |a pelvic mass | |
| 650 | 7 | |a Antigens, Tumor-Associated, Carbohydrate |2 NLM | |
| 650 | 7 | |a Biomarkers, Tumor |2 NLM | |
| 650 | 7 | |a CA-125 Antigen |2 NLM | |
| 650 | 7 | |a Lectins, C-Type |2 NLM | |
| 650 | 7 | |a MGL lectin, human |2 NLM | |
| 650 | 7 | |a MUC16 protein, human |2 NLM | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
| 650 | 7 | |a sialosyl-Tn antigen |2 NLM | |
| 700 | 1 | |a Nadeem, Nimrah |e verfasserin |4 aut | |
| 700 | 1 | |a Rolfsen, Anne Lone |e verfasserin |4 aut | |
| 700 | 1 | |a Dørum, Anne |e verfasserin |4 aut | |
| 700 | 1 | |a Laajala, Teemu D |e verfasserin |4 aut | |
| 700 | 1 | |a Grènman, Seija |e verfasserin |4 aut | |
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| 700 | 1 | |a Heinosalo, Taija |e verfasserin |4 aut | |
| 700 | 1 | |a Perheentupa, Antti |e verfasserin |4 aut | |
| 700 | 1 | |a Poutanen, Matti |e verfasserin |4 aut | |
| 700 | 1 | |a Bolstad, Nils |e verfasserin |4 aut | |
| 700 | 1 | |a Carpén, Olli |e verfasserin |4 aut | |
| 700 | 1 | |a Lamminmäki, Urpo |e verfasserin |4 aut | |
| 700 | 1 | |a Pettersson, Kim |e verfasserin |4 aut | |
| 700 | 1 | |a Gidwani, Kamlesh |e verfasserin |4 aut | |
| 700 | 1 | |a Hynninen, Johanna |e verfasserin |4 aut | |
| 700 | 1 | |a Huhtinen, Kaisa |e verfasserin |4 aut | |
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