How to orient cells in microcavities for high resolution imaging of cytokinesis and lumen formation
© 2020 Elsevier Inc. All rights reserved..
Imaging dynamics of cellular morphogenesis with high spatial-temporal resolution in 3D is challenging, due to the low spatial resolution along the optical axis and photo-toxicity. However, some cellular structures are planar and hence 2D imaging should be sufficient, provided that the structure of interest can be oriented with respect to the optical axis of the microscope. Here, we report a 3D microfabrication method which positions and orients cell divisions very close to the microscope coverglass. We use this approach to study cytokinesis in fission yeasts and polarization to lumen formation in mammalian epithelial cells. We show that this method improves spatial resolution on range of common microscopies, including super-resolution STED. Altogether, this method could shed new lights on self-organization phenomena in single cells and 3D cell culture systems.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:158 |
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| Enthalten in: |
Methods in cell biology - 158(2020) vom: 16., Seite 25-41 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bhat, Alka [VerfasserIn] |
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| Links: |
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| Themen: |
Biological physics |
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| Anmerkungen: |
Date Completed 08.07.2021 Date Revised 08.07.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/bs.mcb.2020.01.002 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM310062470 |
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| 520 | |a © 2020 Elsevier Inc. All rights reserved. | ||
| 520 | |a Imaging dynamics of cellular morphogenesis with high spatial-temporal resolution in 3D is challenging, due to the low spatial resolution along the optical axis and photo-toxicity. However, some cellular structures are planar and hence 2D imaging should be sufficient, provided that the structure of interest can be oriented with respect to the optical axis of the microscope. Here, we report a 3D microfabrication method which positions and orients cell divisions very close to the microscope coverglass. We use this approach to study cytokinesis in fission yeasts and polarization to lumen formation in mammalian epithelial cells. We show that this method improves spatial resolution on range of common microscopies, including super-resolution STED. Altogether, this method could shed new lights on self-organization phenomena in single cells and 3D cell culture systems | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Biological physics | |
| 650 | 4 | |a Cell polarization | |
| 650 | 4 | |a Cytokinesis | |
| 650 | 4 | |a Microfabrication | |
| 650 | 4 | |a Organoids | |
| 650 | 4 | |a Super-resolution STED | |
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| 700 | 1 | |a Lu, Linjie |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Chen-Ho |e verfasserin |4 aut | |
| 700 | 1 | |a Lo Vecchio, Simon |e verfasserin |4 aut | |
| 700 | 1 | |a Maraspini, Riccardo |e verfasserin |4 aut | |
| 700 | 1 | |a Honigmann, Alf |e verfasserin |4 aut | |
| 700 | 1 | |a Riveline, Daniel |e verfasserin |4 aut | |
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