Simple autofocusing method by image processing using transmission images for large-scale two-photon lithography
We propose a simple autofocusing technique that can be introduced into conventional two-photon lithography systems without additional devices. Autofocusing is achieved by image processing using transmission images of photopolymerized voxels. The signal-to-noise ratio of transmission images was improved by optimal low-pass filtering to detect voxels in them. The focal point was detected with an accuracy of about 250 nm from the difference images. Further, we demonstrated mass-fabrication of a 5 × 5 spiral square array with an area of 900 × 900 µm2 using this method. The method has potential application in constructing low-cost, compact and versatile two-photon lithography apparatus.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Optics express - 28(2020), 8 vom: 13. Apr., Seite 12342-12351 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fujishiro, Yoko [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Revised 14.05.2020 published: Print Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1364/OE.390486 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM309866987 |
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| 520 | |a We propose a simple autofocusing technique that can be introduced into conventional two-photon lithography systems without additional devices. Autofocusing is achieved by image processing using transmission images of photopolymerized voxels. The signal-to-noise ratio of transmission images was improved by optimal low-pass filtering to detect voxels in them. The focal point was detected with an accuracy of about 250 nm from the difference images. Further, we demonstrated mass-fabrication of a 5 × 5 spiral square array with an area of 900 × 900 µm2 using this method. The method has potential application in constructing low-cost, compact and versatile two-photon lithography apparatus | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Maruo, Shoji |e verfasserin |4 aut | |
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