Molecular Cytogenetic Analysis of Karyotype and Y Chromosome Conservation in Species of the Genus Talpa (Insectivora)

© 2020 S. Karger AG, Basel..

The Talpidae family has a highly stable karyotype. Most of the chromosome studies in this mammal group, however, employed classical cytogenetic techniques. Molecular cytogenetic analyses are still scarce and, for example, no repeated DNA sequences have been described to date. In this work, we used sequence analysis, chromosomal mapping of a LINE1 retroelement sequence, as well as chromosome painting with a whole Y chromosome probe of T. occidentalis to compare the karyotypes of 3 species of the genus Talpa (T. occidentalis, T. romana, and T. aquitania). Our results demonstrate that in Talpa genomes LINE1 sequences are widely distributed on all chromosomes but are enriched in pericentromeric C-band-positive regions. In addition, these LINE1 accumulate on the Y chromosomes of the 3 Talpa species regardless of their euchromatic or heterochromatic condition. Chromosome painting shows that the Y chromosomes in these 3 species are highly conserved. Interestingly, they share sequences with heterochromatic blocks on chromosome pairs 14 and 16 and, to a lesser degree, with the pericentromeric regions of other autosomes. Together, our analyses demonstrate that the repetitive DNA content of chromosomes from Talpa species is highly conserved.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:160

Enthalten in:

Cytogenetic and genome research - 160(2020), 5 vom: 11., Seite 264-271

Sprache:

Englisch

Beteiligte Personen:

Gutierrez, Juana [VerfasserIn]
Aleix-Mata, Gael [VerfasserIn]
Marchal, Juan A [VerfasserIn]
Arroyo, María [VerfasserIn]
Castiglia, Riccardo [VerfasserIn]
Sánchez, Antonio [VerfasserIn]

Links:

Volltext

Themen:

Talpa
Heterochromatin
Journal Article
LINE1
Repeat sequences
Y chromosome

Anmerkungen:

Date Completed 16.09.2020

Date Revised 04.12.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1159/000507836

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM309799708