Neuroprotective mechanisms of ε-viniferin in a rotenone-induced cell model of Parkinson's disease : significance of SIRT3-mediated FOXO3 deacetylation

Trans-(-)-ε-viniferin (ε-viniferin) has antioxidative and anti-inflammatory effects. It also has neuroprotective effects in Huntington's disease by activating the SIRT3/LKB1/AMPK signaling pathway; however, it remains unknown whether ε-viniferin also has a neuroprotective role in Parkinson's disease. A Parkinson's disease cell model was induced by exposing SH-SY5Y cells to 3.0 μM rotenone for 24 hours, and cells were then treated with 1.0 μM ε-viniferin for 24 hours. Treatment with ε-viniferin upregulated SIRT3 expression, which promoted FOXO3 deacetylation and nuclear localization. ε-Viniferin also increased ATP production and decreased reactive oxygen species production. Furthermore, ε-viniferin treatment alleviated rotenone-induced mitochondrial depolarization and reduced cell apoptosis, and restored the expression of mitochondrial homeostasis-related proteins. However, when cells were transfected with SIRT3 or FOXO3 shRNA prior to rotenone and ε-viniferin treatment, these changes were reversed. The results from the present study indicate that ε-viniferin enhances SIRT3-mediated FOXO3 deacetylation, reduces oxidative stress, and maintains mitochondrial homeostasis, thus inhibiting rotenone-induced cell apoptosis. ε-Viniferin may therefore be a promising treatment strategy for Parkinson's disease.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:15

Enthalten in:

Neural regeneration research - 15(2020), 11 vom: 12. Nov., Seite 2143-2153

Sprache:

Englisch

Beteiligte Personen:

Zhang, Shuo [VerfasserIn]
Ma, Yan [VerfasserIn]
Feng, Juan [VerfasserIn]

Links:

Volltext

Themen:

ε-viniferin
Deacetylation
FOXO3
Journal Article
Mitochondrial homeostasis
Mitophagy
Oxidative stress
Parkinson’s disease
SIRT3

Anmerkungen:

Date Revised 11.12.2020

published: Print

Citation Status PubMed-not-MEDLINE

doi:

10.4103/1673-5374.282264

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM309781043