Bacterial riboproteogenomics : the era of N-terminal proteoform existence revealed
© FEMS 2020..
With the rapid increase in the number of sequenced prokaryotic genomes, relying on automated gene annotation became a necessity. Multiple lines of evidence, however, suggest that current bacterial genome annotations may contain inconsistencies and are incomplete, even for so-called well-annotated genomes. We here discuss underexplored sources of protein diversity and new methodologies for high-throughput genome reannotation. The expression of multiple molecular forms of proteins (proteoforms) from a single gene, particularly driven by alternative translation initiation, is gaining interest as a prominent contributor to bacterial protein diversity. In consequence, riboproteogenomic pipelines were proposed to comprehensively capture proteoform expression in prokaryotes by the complementary use of (positional) proteomics and the direct readout of translated genomic regions using ribosome profiling. To complement these discoveries, tailored strategies are required for the functional characterization of newly discovered bacterial proteoforms.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
FEMS microbiology reviews - 44(2020), 4 vom: 01. Juli, Seite 418-431 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fijalkowska, Daria [VerfasserIn] |
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| Links: |
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| Themen: |
(alternative) translation initiation |
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| Anmerkungen: |
Date Completed 24.02.2021 Date Revised 24.02.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/femsre/fuaa013 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM309694167 |
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| 520 | |a © FEMS 2020. | ||
| 520 | |a With the rapid increase in the number of sequenced prokaryotic genomes, relying on automated gene annotation became a necessity. Multiple lines of evidence, however, suggest that current bacterial genome annotations may contain inconsistencies and are incomplete, even for so-called well-annotated genomes. We here discuss underexplored sources of protein diversity and new methodologies for high-throughput genome reannotation. The expression of multiple molecular forms of proteins (proteoforms) from a single gene, particularly driven by alternative translation initiation, is gaining interest as a prominent contributor to bacterial protein diversity. In consequence, riboproteogenomic pipelines were proposed to comprehensively capture proteoform expression in prokaryotes by the complementary use of (positional) proteomics and the direct readout of translated genomic regions using ribosome profiling. To complement these discoveries, tailored strategies are required for the functional characterization of newly discovered bacterial proteoforms | ||
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| 700 | 1 | |a Willems, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Van Damme, Petra |e verfasserin |4 aut | |
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