Differential expression pattern of the proteome in response to cadmium stress based on proteomics analysis of wheat roots
BACKGROUND: Heavy metal cadmium (Cd) is a common environmental pollutant in soils, which has an negative impacts on crop growth and development. At present, cadmium has become a major soil and water heavy metal pollutant, which not only causes permanent and irreversible health problems for humans, but also causes a significant reduction in crop yields.
RESULTS: This study examined the chemical forms of Cd in the roots of two wheat varieties (M1019 and Xinong20) by continuous extraction and analyzed differences in distribution characteristics of Cd in the root cell wall, cytoplasm, and organelles by elemental content determination and subcellular separation. Furthermore, we conducted proteomics analysis of the roots of the two varieties under Cd pollution using mass spectrometry quantitative proteomics techniques. A total of 11,651 proteins were identified, of which 10,532 proteins contained quantitative information. In addition, the differentially expressed proteins in the two varieties were related to DNA replication and repair, protein metabolism, and the glutathione metabolism pathway.
CONCLUSION: The results of this study improve our understanding of the mechanism of plant responses to Cd stress.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
|---|---|
| Enthalten in: |
BMC genomics - 21(2020), 1 vom: 07. Mai, Seite 343 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Jian, Mingyang [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
00BH33GNGH |
|---|
| Anmerkungen: |
Date Completed 11.01.2021 Date Revised 11.01.2021 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1186/s12864-020-6716-8 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM309642175 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM309642175 | ||
| 003 | DE-627 | ||
| 005 | 20231225134350.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1186/s12864-020-6716-8 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1032.xml |
| 035 | |a (DE-627)NLM309642175 | ||
| 035 | |a (NLM)32380942 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Jian, Mingyang |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Differential expression pattern of the proteome in response to cadmium stress based on proteomics analysis of wheat roots |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 11.01.2021 | ||
| 500 | |a Date Revised 11.01.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Heavy metal cadmium (Cd) is a common environmental pollutant in soils, which has an negative impacts on crop growth and development. At present, cadmium has become a major soil and water heavy metal pollutant, which not only causes permanent and irreversible health problems for humans, but also causes a significant reduction in crop yields | ||
| 520 | |a RESULTS: This study examined the chemical forms of Cd in the roots of two wheat varieties (M1019 and Xinong20) by continuous extraction and analyzed differences in distribution characteristics of Cd in the root cell wall, cytoplasm, and organelles by elemental content determination and subcellular separation. Furthermore, we conducted proteomics analysis of the roots of the two varieties under Cd pollution using mass spectrometry quantitative proteomics techniques. A total of 11,651 proteins were identified, of which 10,532 proteins contained quantitative information. In addition, the differentially expressed proteins in the two varieties were related to DNA replication and repair, protein metabolism, and the glutathione metabolism pathway | ||
| 520 | |a CONCLUSION: The results of this study improve our understanding of the mechanism of plant responses to Cd stress | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cd stress | |
| 650 | 4 | |a Chemical forms | |
| 650 | 4 | |a Proteomics analysis | |
| 650 | 4 | |a Wheat roots | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 650 | 7 | |a Proteome |2 NLM | |
| 650 | 7 | |a Soil Pollutants |2 NLM | |
| 650 | 7 | |a Cadmium |2 NLM | |
| 650 | 7 | |a 00BH33GNGH |2 NLM | |
| 700 | 1 | |a Zhang, Dazhong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaoying |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Shuwei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Yue |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Qin |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Yucui |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Lingjian |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t BMC genomics |d 2000 |g 21(2020), 1 vom: 07. Mai, Seite 343 |w (DE-627)NLM10921594X |x 1471-2164 |7 nnns |
| 773 | 1 | 8 | |g volume:21 |g year:2020 |g number:1 |g day:07 |g month:05 |g pages:343 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1186/s12864-020-6716-8 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 21 |j 2020 |e 1 |b 07 |c 05 |h 343 | ||