CRISPR/Cas9-Mediated SlMYC2 Mutagenesis Adverse to Tomato Plant Growth and MeJA-Induced Fruit Resistance to Botrytis cinerea

Methyl jasmonate (MeJA), a natural phytohormone, played a critical role not only in plant growth but also in plant defense response to biotic and abiotic stresses. MYC2, a basic helix-loop-helix transcription factor, is a master regulator in MeJA signaling pathway. In the present work, slmyc2 mutants were generated by the clustered regularly interspaced short palindromic repeats and associated Cas9 protein (CRISPR/Cas9) system to investigate the role of SlMYC2 in tomato plant growth and fruit disease resistance induced by exogenous MeJA. The results showed that slmyc2 mutants possessed a higher number of flowers and a lower fruit setting rate in comparison with wild-type plants. In addition, the fruit shape of slmyc2 mutant was prolate, while the control fruits were oblate. Knockout of SlMYC2 significantly decreased the activities of disease defensive and antioxidant enzymes, as well as the expression levels of pathogen-related (PR) genes (SlPR-1 and SlPR-STH2) and the key genes related to jasmonic acid (JA) biosynthesis and signaling pathway including allene oxide cyclase (SlAOC), lipoxygenase D (SlLOXD), SlMYC2, and coronatine insensitive 1 (SlCOI1), and consequently aggravated the disease symptoms. By contrast, the disease symptoms were largely reduced in MeJA-treated fruit that possessed higher activities of these enzymes and expression levels of genes. However, the induction effects of MeJA on fruit disease resistance and these enzymes' activities and genes' expressions were significantly attenuated by knockout of SlMYC2. Therefore, the results indicated that SlMYC2 played positive regulatory roles not only in the growth of tomato plants but also in MeJA-induced disease resistance and the antioxidant process in tomato fruits.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:68

Enthalten in:

Journal of agricultural and food chemistry - 68(2020), 20 vom: 20. Mai, Seite 5529-5538

Sprache:

Englisch

Beteiligte Personen:

Shu, Pan [VerfasserIn]
Li, Ziye [VerfasserIn]
Min, Dedong [VerfasserIn]
Zhang, Xinhua [VerfasserIn]
Ai, Wen [VerfasserIn]
Li, Jiaozhuo [VerfasserIn]
Zhou, Jingxiang [VerfasserIn]
Li, Zilong [VerfasserIn]
Li, Fujun [VerfasserIn]
Li, Xiaoan [VerfasserIn]

Links:

Volltext

Themen:

900N171A0F
Acetates
Botrytis cinerea
CRISPR/Cas9
Cyclopentanes
Journal Article
MYC2
Methyl jasmonate
Oxylipins
Plant Growth Regulators
Plant Proteins
Tomato fruit

Anmerkungen:

Date Completed 06.01.2021

Date Revised 07.12.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1021/acs.jafc.9b08069

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM309560713