UNC5B mediates G2/M phase arrest of bladder cancer cells by binding to CDC14A and P53
UNC5B is a known tumor suppressor gene in a variety of cancers. As a transmembrane protein, UNC5B also induces apoptosis in a P53-dependent manner. In this study, we demonstrate that UNC5B inhibits proliferation through G2/M phase arrest by mass spectrometry and bioinformatics analysis in bladder cancer cells. By combing with CDC14A and P53, UNC5B dephosphorylated P53 at Ser-315 site. This dephosphorylation facilitated G2/M phase arrest by reducing the expression of cyclin B1 and increasing the expression of p-CDK1, thus inhibiting tumor proliferation. Knockdown of CDC14A suppressed the G2/M phase arrest induced by UNC5B in vitro, and eliminated the inhibitory effect of UNC5B on tumor proliferation in vivo. Our results show that UNC5B-mediated cell cycle arrest may act as a potential treatment for bladder cancer.
| Errataetall: |
ErratumIn: Cancer Gene Ther. 2022 Mar;29(3-4):398. - PMID 34671114 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:27 |
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| Enthalten in: |
Cancer gene therapy - 27(2020), 12 vom: 05. Dez., Seite 934-947 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huang, Yexiang [VerfasserIn] |
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| Links: |
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| Themen: |
CDC14A protein, human |
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| Anmerkungen: |
Date Completed 21.12.2021 Date Revised 22.04.2022 published: Print-Electronic ErratumIn: Cancer Gene Ther. 2022 Mar;29(3-4):398. - PMID 34671114 Citation Status MEDLINE |
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| doi: |
10.1038/s41417-020-0175-x |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM30955456X |
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| 520 | |a UNC5B is a known tumor suppressor gene in a variety of cancers. As a transmembrane protein, UNC5B also induces apoptosis in a P53-dependent manner. In this study, we demonstrate that UNC5B inhibits proliferation through G2/M phase arrest by mass spectrometry and bioinformatics analysis in bladder cancer cells. By combing with CDC14A and P53, UNC5B dephosphorylated P53 at Ser-315 site. This dephosphorylation facilitated G2/M phase arrest by reducing the expression of cyclin B1 and increasing the expression of p-CDK1, thus inhibiting tumor proliferation. Knockdown of CDC14A suppressed the G2/M phase arrest induced by UNC5B in vitro, and eliminated the inhibitory effect of UNC5B on tumor proliferation in vivo. Our results show that UNC5B-mediated cell cycle arrest may act as a potential treatment for bladder cancer | ||
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