Stem Cell Research Lab Resource : Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats
Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved..
We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:45 |
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| Enthalten in: |
Stem cell research - 45(2020) vom: 05. Mai, Seite 101827 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Khurana, Preeti [VerfasserIn] |
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| Links: |
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| Themen: |
Atopic dermatitis |
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| Anmerkungen: |
Date Completed 21.06.2021 Date Revised 31.05.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.scr.2020.101827 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM309449235 |
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| 500 | |a Date Revised 31.05.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Atopic dermatitis | |
| 650 | 4 | |a Filaggrin | |
| 650 | 4 | |a IPSC line | |
| 650 | 4 | |a Keratinocyte | |
| 650 | 4 | |a Pluripotency | |
| 650 | 7 | |a FLG protein, human |2 NLM | |
| 650 | 7 | |a Filaggrin Proteins |2 NLM | |
| 650 | 7 | |a Intermediate Filament Proteins |2 NLM | |
| 700 | 1 | |a Kolundzic, Nikola |e verfasserin |4 aut | |
| 700 | 1 | |a Rogar, Marija |e verfasserin |4 aut | |
| 700 | 1 | |a Hobbs, Carl |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, X F Colin C |e verfasserin |4 aut | |
| 700 | 1 | |a Common, John E A |e verfasserin |4 aut | |
| 700 | 1 | |a Ilic, Dusko |e verfasserin |4 aut | |
| 700 | 1 | |a Liovic, Mirjana |e verfasserin |4 aut | |
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