Quantification of microbiota-related phenols and aromatic acids in mouse feces of a diabetic nephropathy model by simultaneous BDAPE derivatization using ultra-performance liquid chromatography-tandem mass spectrometry
In the intestine, several phenols and aromatic acids are generated by microbiota and are highly related to the formation of uremic toxins. Herein, we developed a new derivatization reagent, 2-bromo-1-[4-(dimethylamino)phenyl] ethyl ketone (BDAPE), that reacted simultaneously with phenols and aromatic acids. Following a reaction within 2 h at 60 °C in the presence of 200 mM potassium carbonate (K2CO3), the obtained BDAPE derivatives were separated on a reversed-phase C18 column and quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in positive electrospray ionization mode. This method allowed a lower limit of quantification (LLOQ) of 0.090 μΜ for 3-indolepropionic acid (3IPA), indole-3-acetic acid (3IAA), p-cresol (PC), benzoic acid (BA), and phenol (PN); 0.30 μΜ for phenylacetic acid (PAA); 0.45 μΜ for 4-hydroxyphenylacetic acid (4HPAA); and 0.60 μΜ for 3-phenylpropionic acid (PPA). Methodological validation further demonstrated acceptable accuracy (%RE < 16.1) and precision (%RSD < 16.2), suggesting that this is a sensitive and robust method for simultaneous quantification of phenols and aromatic acids. The method was successfully applied to analyze these microbiota-related analytes in mouse feces of a diabetic nephropathy model. Graphical abstract.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:412 |
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| Enthalten in: |
Analytical and bioanalytical chemistry - 412(2020), 13 vom: 05. Mai, Seite 3241-3252 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zheng, Xiaoli [VerfasserIn] |
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| Links: |
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| Themen: |
2-Bromo-1-[4-(dimethylamino)phenyl] ethyl ketone |
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| Anmerkungen: |
Date Completed 29.01.2021 Date Revised 29.01.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00216-020-02585-w |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM30926152X |
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| 245 | 1 | 0 | |a Quantification of microbiota-related phenols and aromatic acids in mouse feces of a diabetic nephropathy model by simultaneous BDAPE derivatization using ultra-performance liquid chromatography-tandem mass spectrometry |
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| 520 | |a In the intestine, several phenols and aromatic acids are generated by microbiota and are highly related to the formation of uremic toxins. Herein, we developed a new derivatization reagent, 2-bromo-1-[4-(dimethylamino)phenyl] ethyl ketone (BDAPE), that reacted simultaneously with phenols and aromatic acids. Following a reaction within 2 h at 60 °C in the presence of 200 mM potassium carbonate (K2CO3), the obtained BDAPE derivatives were separated on a reversed-phase C18 column and quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in positive electrospray ionization mode. This method allowed a lower limit of quantification (LLOQ) of 0.090 μΜ for 3-indolepropionic acid (3IPA), indole-3-acetic acid (3IAA), p-cresol (PC), benzoic acid (BA), and phenol (PN); 0.30 μΜ for phenylacetic acid (PAA); 0.45 μΜ for 4-hydroxyphenylacetic acid (4HPAA); and 0.60 μΜ for 3-phenylpropionic acid (PPA). Methodological validation further demonstrated acceptable accuracy (%RE < 16.1) and precision (%RSD < 16.2), suggesting that this is a sensitive and robust method for simultaneous quantification of phenols and aromatic acids. The method was successfully applied to analyze these microbiota-related analytes in mouse feces of a diabetic nephropathy model. Graphical abstract | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 2-Bromo-1-[4-(dimethylamino)phenyl] ethyl ketone | |
| 650 | 4 | |a Aromatic acid | |
| 650 | 4 | |a Microbiota | |
| 650 | 4 | |a Phenols | |
| 650 | 4 | |a Simultaneous derivatization | |
| 650 | 4 | |a Uremic toxin | |
| 650 | 7 | |a Acids |2 NLM | |
| 650 | 7 | |a Phenols |2 NLM | |
| 700 | 1 | |a Duan, Haonan |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Feifei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xiaomei |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Jianhua |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Fanghui |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Fubao |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Shilin |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Lu |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jia |e verfasserin |4 aut | |
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