Separation of Macrophages and Fibroblasts Using Contactless Dielectrophoresis and a Novel ImageJ Macro
Copyright 2019, Mary Ann Liebert, Inc., publishers..
Background: This study presents a label-free method of separating macrophages and fibroblasts, cell types critically associated with tumors. Materials and Methods: Contactless dielectrophoresis (DEP) devices were used to separate fibroblasts from macrophages by selectively trapping one population. An ImageJ macro was developed to determine the percentage of each population moving or stationary at a given point in time in a video. Results: At 350Vrms, 20 kHz, and 1.25 μL/min, more than 90% of fibroblasts were trapped while less than 20% of macrophages were trapped. Conclusions: Contactless DEP was used to study macrophage and fibroblast separation as a proof-of-concept study for separating cells in the tumor microenvironment. The associated ImageJ macro could be used in other microfluidic cell separation studies.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1 |
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| Enthalten in: |
Bioelectricity - 1(2019), 1 vom: 01. März, Seite 49-55 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Douglas, Temple Anne [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 28.09.2020 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1089/bioe.2018.0004 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM308778650 |
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| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Copyright 2019, Mary Ann Liebert, Inc., publishers. | ||
| 520 | |a Background: This study presents a label-free method of separating macrophages and fibroblasts, cell types critically associated with tumors. Materials and Methods: Contactless dielectrophoresis (DEP) devices were used to separate fibroblasts from macrophages by selectively trapping one population. An ImageJ macro was developed to determine the percentage of each population moving or stationary at a given point in time in a video. Results: At 350Vrms, 20 kHz, and 1.25 μL/min, more than 90% of fibroblasts were trapped while less than 20% of macrophages were trapped. Conclusions: Contactless DEP was used to study macrophage and fibroblast separation as a proof-of-concept study for separating cells in the tumor microenvironment. The associated ImageJ macro could be used in other microfluidic cell separation studies | ||
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| 700 | 1 | |a Schmelz, Eva M |e verfasserin |4 aut | |
| 700 | 1 | |a Davalos, Rafael V |e verfasserin |4 aut | |
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