Graphene quantum dots supported by graphene oxide as a sensitive fluorescence nanosensor for cytochrome c detection and intracellular imaging
A new class of cytochrome c (Cyt c) detection fluorescence sensor (GQDs-GO) based on graphene quantum dots (GQDs) supported by graphene oxide (GO) has been developed through simply mixing a certain amount of GQDs and GO. It is found that fluorescence "turn on" or "turn off" can be adjusted easily according to the concentration of Cyt c. When the concentration of Cyt c is lower than 1.9 μmol L-1, the fluorescence is "turn on", whereas it is "turn off" beyond 1.9 μmol L-1. The process is fast and can be completed within 10 s. By means of UV-vis absorption spectra, it is demonstrated that the changed fluorescence comes from the different interactions among GQDs, GO and Cyt c. Additionally, the GQDs-GO shows a high selectivity for Cyt c detection, and further exhibits favorable intracellular imaging in A549 cells. This study opens a new direction for developing a low-cost, highly efficient, GQDs-based fluorescence sensor for Cyt c detection, which make it very attractive for biological imaging applications.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2017 |
---|---|
Erschienen: |
2017 |
Enthalten in: |
Zur Gesamtaufnahme - volume:5 |
---|---|
Enthalten in: |
Journal of materials chemistry. B - 5(2017), 31 vom: 21. Aug., Seite 6300-6306 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Cao, Lin [VerfasserIn] |
---|
Links: |
---|
Themen: |
---|
Anmerkungen: |
Date Revised 08.04.2020 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
---|
doi: |
10.1039/c7tb01629h |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM308497260 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM308497260 | ||
003 | DE-627 | ||
005 | 20231225131846.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2017 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1039/c7tb01629h |2 doi | |
028 | 5 | 2 | |a pubmed24n1028.xml |
035 | |a (DE-627)NLM308497260 | ||
035 | |a (NLM)32264446 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Cao, Lin |e verfasserin |4 aut | |
245 | 1 | 0 | |a Graphene quantum dots supported by graphene oxide as a sensitive fluorescence nanosensor for cytochrome c detection and intracellular imaging |
264 | 1 | |c 2017 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Revised 08.04.2020 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a A new class of cytochrome c (Cyt c) detection fluorescence sensor (GQDs-GO) based on graphene quantum dots (GQDs) supported by graphene oxide (GO) has been developed through simply mixing a certain amount of GQDs and GO. It is found that fluorescence "turn on" or "turn off" can be adjusted easily according to the concentration of Cyt c. When the concentration of Cyt c is lower than 1.9 μmol L-1, the fluorescence is "turn on", whereas it is "turn off" beyond 1.9 μmol L-1. The process is fast and can be completed within 10 s. By means of UV-vis absorption spectra, it is demonstrated that the changed fluorescence comes from the different interactions among GQDs, GO and Cyt c. Additionally, the GQDs-GO shows a high selectivity for Cyt c detection, and further exhibits favorable intracellular imaging in A549 cells. This study opens a new direction for developing a low-cost, highly efficient, GQDs-based fluorescence sensor for Cyt c detection, which make it very attractive for biological imaging applications | ||
650 | 4 | |a Journal Article | |
700 | 1 | |a Li, Xiangqing |e verfasserin |4 aut | |
700 | 1 | |a Qin, Lixia |e verfasserin |4 aut | |
700 | 1 | |a Kang, Shi-Zhao |e verfasserin |4 aut | |
700 | 1 | |a Li, Guodong |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Journal of materials chemistry. B |d 2013 |g 5(2017), 31 vom: 21. Aug., Seite 6300-6306 |w (DE-627)NLM225907968 |x 2050-7518 |7 nnns |
773 | 1 | 8 | |g volume:5 |g year:2017 |g number:31 |g day:21 |g month:08 |g pages:6300-6306 |
856 | 4 | 0 | |u http://dx.doi.org/10.1039/c7tb01629h |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 5 |j 2017 |e 31 |b 21 |c 08 |h 6300-6306 |