Transplantation of sh-miR-199a-5p-Modified Olfactory Ensheathing Cells Promotes the Functional Recovery in Rats with Contusive Spinal Cord Injury

MicroRNAs (miRNAs) function as gene expression switches, and participate in diverse pathophysiological processes of spinal cord injury (SCI). Olfactory ensheathing cells (OECs) can alleviate pathological injury and facilitate functional recovery after SCI. However, the mechanisms by which OECs restore function are not well understood. This study aims to determine whether silencing miR-199a-5p would enhance the beneficial effects of the OECs. In this study, we measured miR-199a-5p levels in rat spinal cords with and without injury, with and without OEC transplants. Then, we transfected OECs with the sh-miR-199a-5p lentiviral vector to reduce miR-199a-5p expression and determined the effects of these OECs in SCI rats by Basso-Beattie-Bresnahan (BBB) locomotor scores, diffusion tensor imaging (DTI), and histological methods. We used western blotting to measure protein levels of Slit1, Robo2, and srGAP2. Finally, we used the dual-luciferase reporter assay to assess the relationship between miR-199-5p and Slit1, Robo2, and srGAP2 expression. We found that SCI significantly increased miR-199a-5p levels (P < 0.05), and OEC transplants significantly reduced miR-199a-5p expression (P < 0.05). Knockdown of miR-199a-5p in OECs had a better therapeutic effect on SCI rats, indicated by higher BBB scores and fractional anisotropy values on DTI, as well as histological findings. Reducing miR-199a-5p levels in transplanted OECs markedly increased spinal cord protein levels of Slit1, Robo2, and srGAP2. Our results demonstrated that transplantation of sh-miR-199a-5p-modified OECs promoted functional recovery in SCI rats, suggesting that miR-199a-5p knockdown was more beneficial to the therapeutic effects of OEC transplants. These findings provided new insights into miRNAs-mediated therapeutic mechanisms of OECs, which helps us to develop therapeutic strategies based on miRNAs and optimize cell therapy for SCI.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:29

Enthalten in:

Cell transplantation - 29(2020) vom: 01. Jan., Seite 963689720916173

Sprache:

Englisch

Beteiligte Personen:

Gao, Zhengchao [VerfasserIn]
Zhao, Yingjie [VerfasserIn]
He, Xijing [VerfasserIn]
Leng, Zikuan [VerfasserIn]
Zhou, Xiaoqian [VerfasserIn]
Song, Hui [VerfasserIn]
Wang, Rui [VerfasserIn]
Gao, Zhongyang [VerfasserIn]
Wang, Yiqun [VerfasserIn]
Liu, Jiantao [VerfasserIn]
Niu, Binbin [VerfasserIn]
Li, Haopeng [VerfasserIn]
Ouyang, Pengrong [VerfasserIn]
Chang, Su'e [VerfasserIn]

Links:

Volltext

Themen:

Journal Article
MIRN199 microRNA, rat
MiRNAs
MicroRNAs
Nerve regeneration
Olfactory ensheathing cells
Receptors, Immunologic
Research Support, Non-U.S. Gov't
Spinal cord injury

Anmerkungen:

Date Completed 08.06.2021

Date Revised 08.06.2021

published: Print

Citation Status MEDLINE

doi:

10.1177/0963689720916173

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM308378989