Optimized Fluorescent Probe for Specific Imaging of Glutathione S-Transferases in Living Cells and Mice
© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim..
GSTP1 has been considered to be a marker for malignancy in many tissues. However, the existing GST fluorescent probes are unfavorable for in vivo imaging because of the limited emission wavelength or insufficient fluorescence enhancement (six-fold). The limited fluorescence enhancement of GST fluorescent probes is mainly ascribed to the high background signals resulting from the spontaneous reaction between GSH and the probes. In this work, a highly specific GST probe with NIR emission has been successfully developed through optimization of the essential unit of the probe to repress the spontaneous reaction. The novel GST probe exhibits over 100-fold fluorescence enhancement upon incubation with GSTP1/GSH and high selectivity over other potential interference. In addition, the probe has been proved to be capable of tracking endogenous GST in A549 cells. Finally, the in vivo imaging results demonstrate that the probe can be used for effective imaging of endogenous GST activity in subcutaneous tumor mouse with high contrast.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
|---|---|
| Enthalten in: |
Chemistry, an Asian journal - 15(2020), 9 vom: 04. Mai, Seite 1464-1468 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Song, Aiguo [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
EC 2.5.1.18 |
|---|
| Anmerkungen: |
Date Completed 29.12.2020 Date Revised 29.12.2020 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/asia.202000152 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM308132718 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM308132718 | ||
| 003 | DE-627 | ||
| 005 | 20231225131103.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/asia.202000152 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1027.xml |
| 035 | |a (DE-627)NLM308132718 | ||
| 035 | |a (NLM)32227593 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Song, Aiguo |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Optimized Fluorescent Probe for Specific Imaging of Glutathione S-Transferases in Living Cells and Mice |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 29.12.2020 | ||
| 500 | |a Date Revised 29.12.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. | ||
| 520 | |a GSTP1 has been considered to be a marker for malignancy in many tissues. However, the existing GST fluorescent probes are unfavorable for in vivo imaging because of the limited emission wavelength or insufficient fluorescence enhancement (six-fold). The limited fluorescence enhancement of GST fluorescent probes is mainly ascribed to the high background signals resulting from the spontaneous reaction between GSH and the probes. In this work, a highly specific GST probe with NIR emission has been successfully developed through optimization of the essential unit of the probe to repress the spontaneous reaction. The novel GST probe exhibits over 100-fold fluorescence enhancement upon incubation with GSTP1/GSH and high selectivity over other potential interference. In addition, the probe has been proved to be capable of tracking endogenous GST in A549 cells. Finally, the in vivo imaging results demonstrate that the probe can be used for effective imaging of endogenous GST activity in subcutaneous tumor mouse with high contrast | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Fluorescence | |
| 650 | 4 | |a GSTP1 | |
| 650 | 4 | |a Imaging | |
| 650 | 4 | |a Probe | |
| 650 | 4 | |a tumor | |
| 650 | 7 | |a Fluorescent Dyes |2 NLM | |
| 650 | 7 | |a Glutathione Transferase |2 NLM | |
| 650 | 7 | |a EC 2.5.1.18 |2 NLM | |
| 700 | 1 | |a Shen, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Tian |e verfasserin |4 aut | |
| 700 | 1 | |a Gai, Shouchang |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Haiqing |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xinxin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Hui |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Chemistry, an Asian journal |d 2006 |g 15(2020), 9 vom: 04. Mai, Seite 1464-1468 |w (DE-627)NLM169728706 |x 1861-471X |7 nnns |
| 773 | 1 | 8 | |g volume:15 |g year:2020 |g number:9 |g day:04 |g month:05 |g pages:1464-1468 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/asia.202000152 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 15 |j 2020 |e 9 |b 04 |c 05 |h 1464-1468 | ||