The TMEM189 gene encodes plasmanylethanolamine desaturase which introduces the characteristic vinyl ether double bond into plasmalogens
Copyright © 2020 the Author(s). Published by PNAS..
A significant fraction of the glycerophospholipids in the human body is composed of plasmalogens, particularly in the brain, cardiac, and immune cell membranes. A decline in these lipids has been observed in such diseases as Alzheimer's and chronic obstructive pulmonary disease. Plasmalogens contain a characteristic 1-O-alk-1'-enyl ether (vinyl ether) double bond that confers special biophysical, biochemical, and chemical properties to these lipids. However, the genetics of their biosynthesis is not fully understood, since no gene has been identified that encodes plasmanylethanolamine desaturase (E.C. 1.14.99.19), the enzyme introducing the crucial alk-1'-enyl ether double bond. The present work identifies this gene as transmembrane protein 189 (TMEM189). Inactivation of the TMEM189 gene in human HAP1 cells led to a total loss of plasmanylethanolamine desaturase activity, strongly decreased plasmalogen levels, and accumulation of plasmanylethanolamine substrates and resulted in an inability of these cells to form labeled plasmalogens from labeled alkylglycerols. Transient expression of TMEM189 protein, but not of other selected desaturases, recovered this deficit. TMEM189 proteins contain a conserved protein motif (pfam10520) with eight conserved histidines that is shared by an alternative type of plant desaturase but not by other mammalian proteins. Each of these histidines is essential for plasmanylethanolamine desaturase activity. Mice homozygous for an inactivated Tmem189 gene lacked plasmanylethanolamine desaturase activity and had dramatically lowered plasmalogen levels in their tissues. These results assign the TMEM189 gene to plasmanylethanolamine desaturase and suggest that the previously characterized phenotype of Tmem189-deficient mice may be caused by a lack of plasmalogens.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:117 |
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| Enthalten in: |
Proceedings of the National Academy of Sciences of the United States of America - 117(2020), 14 vom: 07. Apr., Seite 7792-7798 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Werner, Ernst R [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 16.07.2020 Date Revised 27.07.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1073/pnas.1917461117 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM307956210 |
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| 100 | 1 | |a Werner, Ernst R |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The TMEM189 gene encodes plasmanylethanolamine desaturase which introduces the characteristic vinyl ether double bond into plasmalogens |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Completed 16.07.2020 | ||
| 500 | |a Date Revised 27.07.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 the Author(s). Published by PNAS. | ||
| 520 | |a A significant fraction of the glycerophospholipids in the human body is composed of plasmalogens, particularly in the brain, cardiac, and immune cell membranes. A decline in these lipids has been observed in such diseases as Alzheimer's and chronic obstructive pulmonary disease. Plasmalogens contain a characteristic 1-O-alk-1'-enyl ether (vinyl ether) double bond that confers special biophysical, biochemical, and chemical properties to these lipids. However, the genetics of their biosynthesis is not fully understood, since no gene has been identified that encodes plasmanylethanolamine desaturase (E.C. 1.14.99.19), the enzyme introducing the crucial alk-1'-enyl ether double bond. The present work identifies this gene as transmembrane protein 189 (TMEM189). Inactivation of the TMEM189 gene in human HAP1 cells led to a total loss of plasmanylethanolamine desaturase activity, strongly decreased plasmalogen levels, and accumulation of plasmanylethanolamine substrates and resulted in an inability of these cells to form labeled plasmalogens from labeled alkylglycerols. Transient expression of TMEM189 protein, but not of other selected desaturases, recovered this deficit. TMEM189 proteins contain a conserved protein motif (pfam10520) with eight conserved histidines that is shared by an alternative type of plant desaturase but not by other mammalian proteins. Each of these histidines is essential for plasmanylethanolamine desaturase activity. Mice homozygous for an inactivated Tmem189 gene lacked plasmanylethanolamine desaturase activity and had dramatically lowered plasmalogen levels in their tissues. These results assign the TMEM189 gene to plasmanylethanolamine desaturase and suggest that the previously characterized phenotype of Tmem189-deficient mice may be caused by a lack of plasmalogens | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a ether lipid | |
| 650 | 4 | |a plasmalogen | |
| 650 | 4 | |a plasmanylethanolamine desaturase | |
| 650 | 4 | |a transmembrane protein 189 | |
| 650 | 7 | |a Lipids |2 NLM | |
| 650 | 7 | |a Plasmalogens |2 NLM | |
| 650 | 7 | |a Vinyl Compounds |2 NLM | |
| 650 | 7 | |a vinyl ether |2 NLM | |
| 650 | 7 | |a 2H2T044E11 |2 NLM | |
| 650 | 7 | |a Oxidoreductases |2 NLM | |
| 650 | 7 | |a EC 1.- |2 NLM | |
| 650 | 7 | |a plasmanylethanolamine desaturase |2 NLM | |
| 650 | 7 | |a EC 1.14.99.19 |2 NLM | |
| 650 | 7 | |a TMEM189 protein, human |2 NLM | |
| 650 | 7 | |a EC 2.3.2.23 |2 NLM | |
| 650 | 7 | |a Ubiquitin-Conjugating Enzymes |2 NLM | |
| 650 | 7 | |a EC 2.3.2.23 |2 NLM | |
| 700 | 1 | |a Keller, Markus A |e verfasserin |4 aut | |
| 700 | 1 | |a Sailer, Sabrina |e verfasserin |4 aut | |
| 700 | 1 | |a Lackner, Katharina |e verfasserin |4 aut | |
| 700 | 1 | |a Koch, Jakob |e verfasserin |4 aut | |
| 700 | 1 | |a Hermann, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Coassin, Stefan |e verfasserin |4 aut | |
| 700 | 1 | |a Golderer, Georg |e verfasserin |4 aut | |
| 700 | 1 | |a Werner-Felmayer, Gabriele |e verfasserin |4 aut | |
| 700 | 1 | |a Zoeller, Raphael A |e verfasserin |4 aut | |
| 700 | 1 | |a Hulo, Nicolas |e verfasserin |4 aut | |
| 700 | 1 | |a Berger, Johannes |e verfasserin |4 aut | |
| 700 | 1 | |a Watschinger, Katrin |e verfasserin |4 aut | |
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