Comparative profiling of exosomal miRNAs in human adult peripheral and umbilical cord blood plasma by deep sequencing

Aim: To assess differential expression profiles of miRNAs in exosomes derived from human peripheral blood (PB) and umbilical cord blood (UCB). Materials & methods: Small RNA sequencing was performed to characterize the miRNA expression in plasma exosomes processed from UCB of five healthy newborns and PB of five normal adult volunteers, and differentially expressed miRNAs were further analyzed. Results: A total of 65 exosomal miRNAs, including 46 upregulated and 19 downregulated, showed differential expression between UCB and PB. Target genes of these miRNAs were mainly enriched in signaling pathways associated with pregnancy, cancers, cell mobility and nervous system. Conclusion: Exosomal miRNAs may have essential roles in the biological functions of UCB, suggesting the therapeutic and biomarker potentials of exosomes in UCB.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:12

Enthalten in:

Epigenomics - 12(2020), 10 vom: 01. Mai, Seite 825-842

Sprache:

Englisch

Beteiligte Personen:

Huang, Sirui [VerfasserIn]
Tang, Zhenlin [VerfasserIn]
Wang, Yuheng [VerfasserIn]
Chen, Danliang [VerfasserIn]
Li, Jinhua [VerfasserIn]
Zhou, Chang [VerfasserIn]
Lu, Xin [VerfasserIn]
Yuan, Yin [VerfasserIn]

Links:

Volltext

Themen:

Exosomal miRNA
Exosomes
Journal Article
MiRNA
MicroRNAs
Next-generation sequencing
Peripheral blood
Plasma
Research Support, Non-U.S. Gov't
Umbilical cord blood

Anmerkungen:

Date Completed 18.08.2021

Date Revised 18.08.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.2217/epi-2019-0213

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM307948986