The effect of polyclonal activators on rabbit B cell antibody production
Copyright © 2020 Elsevier Ltd. All rights reserved..
Stimulation with polyclonal activators is a tool to increase antibody secretion in B cells. The aim of the present study was to select the most effective common commercially available polyclonal activators of rabbit B cells. Specifically, type B oligodeoxynucleotides with unmethylated deoxycytidyl-deoxyguanosine dinucleotides (CpG-ODN), recombinant rabbit interleukin-2 (rrIL-2), lipopolysaccharide (LPS), pokeweed mitogen (PWM) and Resiquimod (R848) were tested on B cells isolated from blood and spleen by fluorescence-activated cell sorting. Based on the obtained data, stimulation with CpG-ODN induced the highest antigen-specific antibody levels detected by ELISA in supernatants when a single activator was used. In contrast, LPS, PWM and R848 showed a weak or no stimulatory effect. Stimulation with a mix of activators was more effective than CpG-ODN alone, which indicates a synergistic effect in the stimulation of antibody production.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:130 |
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| Enthalten in: |
Research in veterinary science - 130(2020) vom: 15. Juni, Seite 193-196 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Stepanova, Hana [VerfasserIn] |
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| Links: |
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| Themen: |
Alpha-Macroglobulins |
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| Anmerkungen: |
Date Completed 10.09.2020 Date Revised 10.09.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.rvsc.2020.03.022 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM307855139 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Stimulation with polyclonal activators is a tool to increase antibody secretion in B cells. The aim of the present study was to select the most effective common commercially available polyclonal activators of rabbit B cells. Specifically, type B oligodeoxynucleotides with unmethylated deoxycytidyl-deoxyguanosine dinucleotides (CpG-ODN), recombinant rabbit interleukin-2 (rrIL-2), lipopolysaccharide (LPS), pokeweed mitogen (PWM) and Resiquimod (R848) were tested on B cells isolated from blood and spleen by fluorescence-activated cell sorting. Based on the obtained data, stimulation with CpG-ODN induced the highest antigen-specific antibody levels detected by ELISA in supernatants when a single activator was used. In contrast, LPS, PWM and R848 showed a weak or no stimulatory effect. Stimulation with a mix of activators was more effective than CpG-ODN alone, which indicates a synergistic effect in the stimulation of antibody production | ||
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| 700 | 1 | |a Machat, Radek |e verfasserin |4 aut | |
| 700 | 1 | |a Jeklova, Edita |e verfasserin |4 aut | |
| 700 | 1 | |a Faldyna, Martin |e verfasserin |4 aut | |
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