Delineating the role of membrane blebs in a hybrid mode of cancer cell invasion in three-dimensional environments
© 2020. Published by The Company of Biologists Ltd..
The study of cancer cell invasion in 3D environments in vitro has revealed a variety of invasive modes, including amoeboid migration, characterized by primarily round cells that invade in a protease- and adhesion-independent manner. Here, we delineate a contractility-dependent migratory mode of primarily round breast cancer cells that is associated with extensive integrin-mediated extracellular matrix (ECM) reorganization that occurs at membrane blebs, with bleb necks sites of integrin clustering and integrin-dependent ECM alignment. We show that the spatiotemporal distribution of blebs and their utilization for ECM reorganization is mediated by functional β1 integrin receptors and other components of focal adhesions. Taken together, the work presented here characterizes a migratory mode of primarily round cancer cells in complex 3D environments and reveals a fundamentally new function for membrane blebs in cancer cell invasion.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:133 |
|---|---|
| Enthalten in: |
Journal of cell science - 133(2020), 8 vom: 28. Apr. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Guzman, Asja [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Cancer |
|---|
| Anmerkungen: |
Date Completed 18.04.2023 Date Revised 29.08.2023 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1242/jcs.236778 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM307797473 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM307797473 | ||
| 003 | DE-627 | ||
| 005 | 20231225130340.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1242/jcs.236778 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1025.xml |
| 035 | |a (DE-627)NLM307797473 | ||
| 035 | |a (NLM)32193332 | ||
| 035 | |a (PII)jcs236778 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Guzman, Asja |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Delineating the role of membrane blebs in a hybrid mode of cancer cell invasion in three-dimensional environments |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 18.04.2023 | ||
| 500 | |a Date Revised 29.08.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020. Published by The Company of Biologists Ltd. | ||
| 520 | |a The study of cancer cell invasion in 3D environments in vitro has revealed a variety of invasive modes, including amoeboid migration, characterized by primarily round cells that invade in a protease- and adhesion-independent manner. Here, we delineate a contractility-dependent migratory mode of primarily round breast cancer cells that is associated with extensive integrin-mediated extracellular matrix (ECM) reorganization that occurs at membrane blebs, with bleb necks sites of integrin clustering and integrin-dependent ECM alignment. We show that the spatiotemporal distribution of blebs and their utilization for ECM reorganization is mediated by functional β1 integrin receptors and other components of focal adhesions. Taken together, the work presented here characterizes a migratory mode of primarily round cancer cells in complex 3D environments and reveals a fundamentally new function for membrane blebs in cancer cell invasion | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Cancer | |
| 650 | 4 | |a Cell migration | |
| 650 | 4 | |a ECM reorganization | |
| 650 | 4 | |a Integrin | |
| 650 | 4 | |a Membrane bleb | |
| 650 | 7 | |a Integrin beta1 |2 NLM | |
| 700 | 1 | |a Avard, Rachel C |e verfasserin |4 aut | |
| 700 | 1 | |a Devanny, Alexander J |e verfasserin |4 aut | |
| 700 | 1 | |a Kweon, Oh Sang |e verfasserin |4 aut | |
| 700 | 1 | |a Kaufman, Laura J |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of cell science |d 1966 |g 133(2020), 8 vom: 28. Apr. |w (DE-627)NLM000100811 |x 1477-9137 |7 nnns |
| 773 | 1 | 8 | |g volume:133 |g year:2020 |g number:8 |g day:28 |g month:04 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1242/jcs.236778 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 133 |j 2020 |e 8 |b 28 |c 04 | ||