Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males
©2020 Byun et al..
This study was carried out to identify and estimate physiological function of a new type of opsin subfamily present in the retina and whole brain tissues of Japanese eel using RNA-Seq transcriptome method. A total of 18 opsin subfamilies were identified through RNA-seq. The visual opsin family included Rh2, SWS2, FWO, DSO, and Exo-Rhod. The non-visual opsin family included four types of melanopsin subfamily (Opn4x1, Opn4x2, Opn4m1, and Opn4m2), peropsin, two types of neuropsin subfamily (Opn5-like, Opn5), Opn3, three types of TMT opsin subfamily (TMT1, 2, 3), VA-opsin, and parapinopsin. In terms of changes in photoreceptor gene expression in the retina of sexually mature and immature male eels, DSO mRNA increased in the maturation group. Analysis of expression of opsin family gene in male eel brain before and after maturation revealed that DSO and SWS2 expression in terms of visual opsin mRNA increased in the sexually mature group. In terms of non-visual opsin mRNA, parapinopsin mRNA increased whereas that of TMT2 decreased in the fore-brain of the sexually mature group. The mRNA for parapinopsin increased in the mid-brain of the sexually mature group, whereas those of TMT1 and TMT3 increased in the hind-brain of the sexually mature group. DSO mRNA also increased in the retina after sexual maturation, and DSO and SWS2 mRNA increased in whole brain part, suggesting that DSO and SWS2 are closely related to sexual maturation.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
|---|---|
| Enthalten in: |
PeerJ - 8(2020) vom: 15., Seite e8326 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Byun, Jun-Hwan [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Anguilla japonica |
|---|
| Anmerkungen: |
Date Revised 28.09.2020 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.7717/peerj.8326 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM307373983 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM307373983 | ||
| 003 | DE-627 | ||
| 005 | 20231225125425.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.7717/peerj.8326 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1024.xml |
| 035 | |a (DE-627)NLM307373983 | ||
| 035 | |a (NLM)32149019 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Byun, Jun-Hwan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 28.09.2020 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a ©2020 Byun et al. | ||
| 520 | |a This study was carried out to identify and estimate physiological function of a new type of opsin subfamily present in the retina and whole brain tissues of Japanese eel using RNA-Seq transcriptome method. A total of 18 opsin subfamilies were identified through RNA-seq. The visual opsin family included Rh2, SWS2, FWO, DSO, and Exo-Rhod. The non-visual opsin family included four types of melanopsin subfamily (Opn4x1, Opn4x2, Opn4m1, and Opn4m2), peropsin, two types of neuropsin subfamily (Opn5-like, Opn5), Opn3, three types of TMT opsin subfamily (TMT1, 2, 3), VA-opsin, and parapinopsin. In terms of changes in photoreceptor gene expression in the retina of sexually mature and immature male eels, DSO mRNA increased in the maturation group. Analysis of expression of opsin family gene in male eel brain before and after maturation revealed that DSO and SWS2 expression in terms of visual opsin mRNA increased in the sexually mature group. In terms of non-visual opsin mRNA, parapinopsin mRNA increased whereas that of TMT2 decreased in the fore-brain of the sexually mature group. The mRNA for parapinopsin increased in the mid-brain of the sexually mature group, whereas those of TMT1 and TMT3 increased in the hind-brain of the sexually mature group. DSO mRNA also increased in the retina after sexual maturation, and DSO and SWS2 mRNA increased in whole brain part, suggesting that DSO and SWS2 are closely related to sexual maturation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Anguilla japonica | |
| 650 | 4 | |a Japanese eel | |
| 650 | 4 | |a Opsin | |
| 650 | 4 | |a Photoreceptor | |
| 650 | 4 | |a Sex maturation | |
| 700 | 1 | |a Hyeon, Ji-Yeon |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Eun-Su |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Byeong-Hoon |e verfasserin |4 aut | |
| 700 | 1 | |a Miyanishi, Hiroshi |e verfasserin |4 aut | |
| 700 | 1 | |a Kagawa, Hirohiko |e verfasserin |4 aut | |
| 700 | 1 | |a Takeuchi, Yuki |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Se-Jae |e verfasserin |4 aut | |
| 700 | 1 | |a Takemura, Akihiro |e verfasserin |4 aut | |
| 700 | 1 | |a Hur, Sung-Pyo |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PeerJ |d 2013 |g 8(2020) vom: 15., Seite e8326 |w (DE-627)NLM227171578 |x 2167-8359 |7 nnns |
| 773 | 1 | 8 | |g volume:8 |g year:2020 |g day:15 |g pages:e8326 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.7717/peerj.8326 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 8 |j 2020 |b 15 |h e8326 | ||