High-throughput dynamical analysis of dielectrophoretic frequency dispersion of single cells based on deflected flow streamlines

Phenotypic quantification of cells based on their plasma membrane capacitance and cytoplasmic conductivity, as determined by their dielectrophoretic frequency dispersion, is often used as a marker for their biological function. However, due to the prevalence of phenotypic heterogeneity in many biological systems of interest, there is a need for methods capable of determining the dielectrophoretic dispersion of single cells at high throughput and without the need for sample dilution. We present a microfluidic device methodology wherein localized constrictions in the microchannel are used to enhance the field delivered by adjoining planar electrodes, so that the dielectrophoresis level and direction on flow-focused cells can be determined on each traversing cell in a high-throughput manner based on their deflected flow streamlines. Using a sample of human red blood cells diluted to 2.25 × 108 cells/mL, the dielectrophoretic translation of single cells traversing at a flow rate of 1.68 μL/min is measured at a throughput of 1.1 × 105 cells/min, to distinguish positive versus negative dielectrophoresis and determine their crossover frequency in media of differing conductivity for validation of the computed membrane capacitance to that from prior methods. We envision application of this dynamic dielectrophoresis (Dy-DEP) method towards high-throughput measurement of the dielectric dispersion of single cells to stratify phenotypic heterogeneity of a particular sample based on their DEP crossover frequency, without the need for significant sample dilution. Grapical abstract.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:412

Enthalten in:

Analytical and bioanalytical chemistry - 412(2020), 16 vom: 03. Juni, Seite 3847-3857

Sprache:

Englisch

Beteiligte Personen:

Torres-Castro, Karina [VerfasserIn]
Honrado, Carlos [VerfasserIn]
Varhue, Walter B [VerfasserIn]
Farmehini, Vahid [VerfasserIn]
Swami, Nathan S [VerfasserIn]

Links:

Volltext

Themen:

Cytometry
Dielectrophoresis
Journal Article
Membrane capacitance
Microfluidics
Phenotype

Anmerkungen:

Date Completed 29.01.2021

Date Revised 02.06.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1007/s00216-020-02467-1

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM307199789