Genomic and biochemical evidence of dietary adaptation in a marine herbivorous fish

Adopting a new diet is a significant evolutionary change, and can profoundly affect an animal's physiology, biochemistry, ecology and genome. To study this evolutionary transition, we investigated the physiology and genomics of digestion of a derived herbivorous fish, Cebidichthys violaceus. We sequenced and assembled its genome (N50 = 6.7 Mb) and digestive transcriptome, and revealed the molecular changes related to digestive enzymes (carbohydrases, proteases and lipases), finding abundant evidence of molecular adaptation. Specifically, two gene families experienced expansion in copy number and adaptive amino acid substitutions: amylase and carboxyl ester lipase (cel), which are involved in the digestion of carbohydrates and lipids, respectively. Both show elevated levels of gene expression and increased enzyme activity. Because carbohydrates are abundant in the prickleback's diet and lipids are rare, these findings suggest that such dietary specialization involves both exploiting abundant resources and scavenging rare ones, especially essential nutrients, like essential fatty acids.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:287

Enthalten in:

Proceedings. Biological sciences - 287(2020), 1921 vom: 26. Feb., Seite 20192327

Sprache:

Englisch

Beteiligte Personen:

Heras, Joseph [VerfasserIn]
Chakraborty, Mahul [VerfasserIn]
Emerson, J J [VerfasserIn]
German, Donovan P [VerfasserIn]

Links:

Volltext

Themen:

Amylase
Carboxyl ester lipase
Digestive physiology
Gene expression
Genome
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Transcriptome

Anmerkungen:

Date Completed 16.06.2020

Date Revised 28.03.2024

published: Print-Electronic

figshare: 10.6084/m9.figshare.c.4838775

Citation Status MEDLINE

doi:

10.1098/rspb.2019.2327

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM306642220