Cyclosporine-a attenuates retinal inflammation by inhibiting HMGB-1 formation in rats with type 2 diabetes mellitus

BACKGROUND: Cyclosporine-A has been regarded as an immunoregulatory and anti-inflammatory drug for the treatment of various immune inflammatory diseases. However, the effect of Cyclosporine-A on the retina of type 2 diabetic rats and the underlying mechanism remains to be elucidated. The objective of the present study was to investigate the effect and mechanism of Cyclosporine-A on diabetic retinopathy.

METHODS: Male Sprague-Dawley rats were established to type 2 diabetic model. After 6 weeks, diabetic rats and normal controls were intravitreally injected with. Cs-A (42 ng/2 μL) to the left eye, and 2 μL DMSO to the right eye for the control.. Another group of normal wild-type rats was subjected to intravitreal injections into. The left eyes with 5 μL PBS or HMGB-1 (5 ng/5 μL) or HMGB-1(5 ng/5 μL) plus. Cs-A (42 ng/2 μL), respectively. Retinal morphological changes were observed with. Hematoxylin-eosin staining. Expressions of HMGB-1, IL-1β and TNF-α were. Detected by immunohistochemistry, ELISA or Western blot or RT-PCR.

RESULTS: Retinal expression levels of IL-1β and TNF-α were upregulated in type 2. diabetic rats and in normal rats with intravitreal injection of HMGB-1, which were. Attenuated by intravitreal Cs-A. Moreover, Cs-A decreased HMGB-1 expression in. diabetic retina and relieved the retinopathy in type 2 diabetic rats.

CONCLUSIONS: Intravitreal administration of Cs-A showed a protective effect on retina. of diabetic rats, possibly by downregulating retinal expressions of IL-1β and TNF-α. via the suppression of HMGB-1.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:21

Enthalten in:

BMC pharmacology & toxicology - 21(2020), 1 vom: 04. Feb., Seite 9

Sprache:

Englisch

Beteiligte Personen:

Wang, Peng [VerfasserIn]
Chen, Fei [VerfasserIn]
Zhang, Xuedong [VerfasserIn]

Links:

Volltext

Themen:

83HN0GTJ6D
Anti-Inflammatory Agents
Cyclosporine
Cyclosporine-a
Diabetic retinopathy
HMGB-1
HMGB1 Protein
Hbp1 protein, rat
IL-1β
IL1B protein, rat
Inflammation
Interleukin-1beta
Journal Article
Research Support, Non-U.S. Gov't
TNF-α
Tumor Necrosis Factor-alpha

Anmerkungen:

Date Completed 04.11.2020

Date Revised 04.11.2020

published: Electronic

Citation Status MEDLINE

doi:

10.1186/s40360-020-0387-6

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM306144158