Cytokine tear film profile determination in eyes of healthy dogs and those with inflammatory periocular and skin disorders
Copyright © 2020 Elsevier B.V. All rights reserved..
Alterations in serum cytokine levels and profiles have been reported in association with a variety of disease conditions (e.g., allergic, immune-mediated, etc.) in both humans and animals. In comparison to serum cytokine measurements, tear cytokine measurements might be expected to more accurately reflect the inflammatory milieu associated with periocular disease. The purpose of this study was to use a multiplexed assay to compare the cytokine profile of tears in healthy dogs to those with inflammatory skin and periocular disease. We were able to detect IL-2, IL-6, IL-8, and TNF-α in >47 % of tear samples from both healthy canine patients and those with inflammatory dermatologic disease (with or without concurrent periocular involvement). In contrast, IL-7, IL-10 and IFN-γ were rarely detected. Dogs with both dermatologic and periocular disease (but not dermatologic disease alone) had higher levels of IL-8 (P < 0.001, P > 0.05, respectively) relative to healthy dogs. Patients with concurrent dermatologic and periocular disease also demonstrated significantly greater variability in IL-8 concentrations between eyes than did healthy dogs (P < 0.0001). Our findings suggest that tear cytokine analysis may prove to be a useful tool to investigate the role and interactions of the local ocular immune response in patients with inflammatory periocular disease.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:221 |
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| Enthalten in: |
Veterinary immunology and immunopathology - 221(2020) vom: 07. März, Seite 110012 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Martinez, P S [VerfasserIn] |
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| Links: |
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| Themen: |
Canine |
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| Anmerkungen: |
Date Completed 16.10.2020 Date Revised 16.10.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.vetimm.2020.110012 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM305753770 |
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| 245 | 1 | 0 | |a Cytokine tear film profile determination in eyes of healthy dogs and those with inflammatory periocular and skin disorders |
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| 500 | |a Date Revised 16.10.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a Alterations in serum cytokine levels and profiles have been reported in association with a variety of disease conditions (e.g., allergic, immune-mediated, etc.) in both humans and animals. In comparison to serum cytokine measurements, tear cytokine measurements might be expected to more accurately reflect the inflammatory milieu associated with periocular disease. The purpose of this study was to use a multiplexed assay to compare the cytokine profile of tears in healthy dogs to those with inflammatory skin and periocular disease. We were able to detect IL-2, IL-6, IL-8, and TNF-α in >47 % of tear samples from both healthy canine patients and those with inflammatory dermatologic disease (with or without concurrent periocular involvement). In contrast, IL-7, IL-10 and IFN-γ were rarely detected. Dogs with both dermatologic and periocular disease (but not dermatologic disease alone) had higher levels of IL-8 (P < 0.001, P > 0.05, respectively) relative to healthy dogs. Patients with concurrent dermatologic and periocular disease also demonstrated significantly greater variability in IL-8 concentrations between eyes than did healthy dogs (P < 0.0001). Our findings suggest that tear cytokine analysis may prove to be a useful tool to investigate the role and interactions of the local ocular immune response in patients with inflammatory periocular disease | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Canine | |
| 650 | 4 | |a Cytokine | |
| 650 | 4 | |a Dermatologic | |
| 650 | 4 | |a Inflammation | |
| 650 | 4 | |a Periocular | |
| 650 | 4 | |a Tear film | |
| 650 | 7 | |a Cytokines |2 NLM | |
| 650 | 7 | |a Interleukin-2 |2 NLM | |
| 650 | 7 | |a Interleukin-6 |2 NLM | |
| 650 | 7 | |a Interleukin-8 |2 NLM | |
| 650 | 7 | |a Tumor Necrosis Factors |2 NLM | |
| 700 | 1 | |a Pucheu, C M |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, C C |e verfasserin |4 aut | |
| 700 | 1 | |a Carter, R T |e verfasserin |4 aut | |
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