Opposing effects of bacterial endophytes on biomass allocation of a wild donor and agricultural recipient
© FEMS 2020..
Root endophytes are a promising tool for increasing plant growth, but it is unclear whether they perform consistently across plant hosts. We characterized the blue grama (Bouteloua gracilis) root microbiome using two sequencing methods, quantified the effects of root endophytes in the original host (blue grama) and an agricultural recipient, corn (Zea mays), under drought and well-watered conditions and examined in vitro mechanisms for plant growth promotion. 16S rRNA amplicon sequencing revealed that the blue grama root microbiome was similar across an elevation gradient, with the exception of four genera. Culturing and Sanger sequencing revealed eight unique endophytes belonging to the genera Bacillus, Lysinibacillus and Pseudomonas. All eight endophytes colonized corn roots, but had opposing effects on aboveground and belowground biomass in each plant species: they increased blue grama shoot mass by 45% (19) (mean +/- SE) while decreasing corn shoot mass by 10% (19), and increased corn root:shoot by 44% (7), while decreasing blue grama root:shoot by 17% (7). Furthermore, contrary to our expectations, endophytes had stronger effects on plant growth under well-watered conditions rather than drought conditions. Collectively, these results suggest that ecological features, including host identity, bacterial traits, climate conditions and morphological outcomes, should be carefully considered in the design and implementation of agricultural inocula.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:96 |
|---|---|
| Enthalten in: |
FEMS microbiology ecology - 96(2020), 3 vom: 01. März |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Rubin, Rachel L [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Bouteloua gracilis |
|---|
| Anmerkungen: |
Date Completed 20.11.2020 Date Revised 20.11.2020 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1093/femsec/fiaa012 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM305589059 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM305589059 | ||
| 003 | DE-627 | ||
| 005 | 20231225121518.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/femsec/fiaa012 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1018.xml |
| 035 | |a (DE-627)NLM305589059 | ||
| 035 | |a (NLM)31960901 | ||
| 035 | |a (PII)fiaa012 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Rubin, Rachel L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Opposing effects of bacterial endophytes on biomass allocation of a wild donor and agricultural recipient |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 20.11.2020 | ||
| 500 | |a Date Revised 20.11.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © FEMS 2020. | ||
| 520 | |a Root endophytes are a promising tool for increasing plant growth, but it is unclear whether they perform consistently across plant hosts. We characterized the blue grama (Bouteloua gracilis) root microbiome using two sequencing methods, quantified the effects of root endophytes in the original host (blue grama) and an agricultural recipient, corn (Zea mays), under drought and well-watered conditions and examined in vitro mechanisms for plant growth promotion. 16S rRNA amplicon sequencing revealed that the blue grama root microbiome was similar across an elevation gradient, with the exception of four genera. Culturing and Sanger sequencing revealed eight unique endophytes belonging to the genera Bacillus, Lysinibacillus and Pseudomonas. All eight endophytes colonized corn roots, but had opposing effects on aboveground and belowground biomass in each plant species: they increased blue grama shoot mass by 45% (19) (mean +/- SE) while decreasing corn shoot mass by 10% (19), and increased corn root:shoot by 44% (7), while decreasing blue grama root:shoot by 17% (7). Furthermore, contrary to our expectations, endophytes had stronger effects on plant growth under well-watered conditions rather than drought conditions. Collectively, these results suggest that ecological features, including host identity, bacterial traits, climate conditions and morphological outcomes, should be carefully considered in the design and implementation of agricultural inocula | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Bouteloua gracilis | |
| 650 | 4 | |a Zea mays | |
| 650 | 4 | |a PGPR | |
| 650 | 4 | |a drought | |
| 650 | 4 | |a endophytes | |
| 650 | 4 | |a rhizobacteria | |
| 650 | 7 | |a RNA, Ribosomal, 16S |2 NLM | |
| 700 | 1 | |a Jones, Ashley N |e verfasserin |4 aut | |
| 700 | 1 | |a Hayer, Michaela |e verfasserin |4 aut | |
| 700 | 1 | |a Shuman-Goodier, Molly E |e verfasserin |4 aut | |
| 700 | 1 | |a Andrews, Lela V |e verfasserin |4 aut | |
| 700 | 1 | |a Hungate, Bruce A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t FEMS microbiology ecology |d 1986 |g 96(2020), 3 vom: 01. März |w (DE-627)NLM088685276 |x 1574-6941 |7 nnns |
| 773 | 1 | 8 | |g volume:96 |g year:2020 |g number:3 |g day:01 |g month:03 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1093/femsec/fiaa012 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 96 |j 2020 |e 3 |b 01 |c 03 | ||