Detection and Preliminary Characterization of Phocine Distemper Virus in a Stranded Harp Seal (Pagophilus groenlandicus) from the Gulf of St. Lawrence, Canada

A lethargic juvenile male harp seal (Pagophilus groenlandicus) in poor nutritional condition was found on the beach on the north shore of Prince Edward Island, Canada, in June 2017. Microscopic examination revealed a severe nonsuppurative encephalitis positive for morbillivirus antigen on immunohistochemistry. Virus isolation attempts were negative. However, phocine distemper virus (PDV) was detected in brain tissue RNA extracts by a seminested reverse transcription PCR that targeted the paramyxovirus RNA-dependent RNA polymerase (pol) gene. Comparison of the resulting partial PDV pol nucleotide sequence revealed it was nearly identical to PDV strains isolated from eastern Atlantic harbor seals (Phoca vitulina vitulina) during a 1988 epizootic in the Wadden and Irish seas, and a western Atlantic harbor seal (Phoca vitulina concolor) that stranded in Maine, US, in 2006. Our study confirmed that closely related PDV strains are circulating in multiple seal species along the coastlines of North America and Europe.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:56

Enthalten in:

Journal of wildlife diseases - 56(2020), 3 vom: 01. Juli, Seite 646-650

Sprache:

Englisch

Beteiligte Personen:

Daoust, Pierre-Yves [VerfasserIn]
Rodrigues, Thaís C S [VerfasserIn]
Shea, Liam B [VerfasserIn]
Subramaniam, Kuttichantran [VerfasserIn]
Waltzek, Thomas B [VerfasserIn]
Nielsen, Ole [VerfasserIn]

Links:

Volltext

Themen:

Case Reports
Harp seal
Immunohistochemistry
Journal Article
Nonsuppurative encephalitis
Pagophilus groenlandicus
Phocine distemper virus
Polymerase chain reaction
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 13.05.2021

Date Revised 13.05.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.7589/2019-10-267

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM305169408