A toolbox for the longitudinal assessment of healthspan in aging mice

The number of people aged over 65 is expected to double in the next 30 years. For many, living longer will mean spending more years with the burdens of chronic diseases such as Alzheimer's disease, cardiovascular disease, and diabetes. Although researchers have made rapid progress in developing geroprotective interventions that target mechanisms of aging and delay or prevent the onset of multiple concurrent age-related diseases, a lack of standardized techniques to assess healthspan in preclinical murine studies has resulted in reduced reproducibility and slow progress. To overcome this, major centers in Europe and the United States skilled in healthspan analysis came together to agree on a toolbox of techniques that can be used to consistently assess the healthspan of mice. Here, we describe the agreed toolbox, which contains protocols for echocardiography, novel object recognition, grip strength, rotarod, glucose tolerance test (GTT) and insulin tolerance test (ITT), body composition, and energy expenditure. The protocols can be performed longitudinally in the same mouse over a period of 4-6 weeks to test how candidate geroprotectors affect cardiac, cognitive, neuromuscular, and metabolic health.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:15

Enthalten in:

Nature protocols - 15(2020), 2 vom: 09. Feb., Seite 540-574

Sprache:

Englisch

Beteiligte Personen:

Bellantuono, I [VerfasserIn]
de Cabo, R [VerfasserIn]
Ehninger, D [VerfasserIn]
Di Germanio, C [VerfasserIn]
Lawrie, A [VerfasserIn]
Miller, J [VerfasserIn]
Mitchell, S J [VerfasserIn]
Navas-Enamorado, I [VerfasserIn]
Potter, P K [VerfasserIn]
Tchkonia, T [VerfasserIn]
Trejo, J L [VerfasserIn]
Lamming, D W [VerfasserIn]

Links:

Volltext

Themen:

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Anmerkungen:

Date Completed 20.04.2020

Date Revised 10.03.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1038/s41596-019-0256-1

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM305147293