Generation of a homozygous HDAC6 knockout human embryonic stem cell line by CRISPR/Cas9 editing
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved..
Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme in the HDAC family. The HDAC6 has been shown to play important roles in several biological processes. Meanwhile, it is also an attractive therapeutic target for a variety of diseases. However, the mechanism of HDAC6 function is not fully understood yet, and it is still lacking highly specific targeted drugs. Here, we generated a homozygous HDAC6 knockout human embryonic stem cell (hESC) line, WAe009-A-21 by the CRISPR/Cas9-based gene editing method. The WAe009-A-21 cell line does not express HDAC6 protein, while maintaining normal 46, XX karyotype, pluripotency, and trilineage differentiation potential.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:41 |
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| Enthalten in: |
Stem cell research - 41(2019) vom: 15. Dez., Seite 101610 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xie, Liangqun [VerfasserIn] |
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| Links: |
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| Themen: |
EC 3.5.1.98 |
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| Anmerkungen: |
Date Completed 01.07.2020 Date Revised 01.07.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.scr.2019.101610 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM303773189 |
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| 500 | |a published: Print-Electronic | ||
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| 520 | |a Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme in the HDAC family. The HDAC6 has been shown to play important roles in several biological processes. Meanwhile, it is also an attractive therapeutic target for a variety of diseases. However, the mechanism of HDAC6 function is not fully understood yet, and it is still lacking highly specific targeted drugs. Here, we generated a homozygous HDAC6 knockout human embryonic stem cell (hESC) line, WAe009-A-21 by the CRISPR/Cas9-based gene editing method. The WAe009-A-21 cell line does not express HDAC6 protein, while maintaining normal 46, XX karyotype, pluripotency, and trilineage differentiation potential | ||
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| 650 | 7 | |a Histone Deacetylase 6 |2 NLM | |
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| 700 | 1 | |a Huang, Jingrui |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xun |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Xinxiu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jiejie |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Jiefeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Weishe |e verfasserin |4 aut | |
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