Assays for Monitoring Toxoplasma gondii Infectivity in the Laboratory Mouse

Toxoplasma is a widespread parasite of animals including many rodents that are a natural part of the transmission cycle between cats, which serve as the definitive host. Although wild rodents, including house mice, are relatively resistant, laboratory mice are highly susceptible to infection. As such, laboratory mice have been used to compare pathogenesis of natural variants and to evaluate the contributions of both host and parasite genes to infection. Protocols are provided here for evaluating acute and chronic infection with different parasite strains in laboratory mice. These protocols should provide uniform standards for evaluating natural variants and attenuated mutants and for comparing outcomes across different studies and between different laboratories.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:2071

Enthalten in:

Methods in molecular biology (Clifton, N.J.) - 2071(2020) vom: 01., Seite 99-116

Sprache:

Englisch

Beteiligte Personen:

Wang, Qiuling [VerfasserIn]
Sibley, L David [VerfasserIn]

Links:

Volltext

Themen:

Bioluminescence
Central nervous system
Dissemination
Journal Article
Pathogenesis
Protozoan Proteins
Research Support, N.I.H., Extramural
Virulence

Anmerkungen:

Date Completed 11.01.2021

Date Revised 16.12.2023

published: Print

Citation Status MEDLINE

doi:

10.1007/978-1-4939-9857-9_5

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM303609842