Discovery and characterization of a stable lipase with preference toward long-chain fatty acids
OBJECTIVES: To identify novel lipases with stability and long-chain fatty acids preference by phylogenetic evolution analysis methods from database.
RESULTS: Thermo-stable Candida antarctica Lipase-A (CALA) was set as a template for gene mining by PSI-BLAST. Based on phylogenetic analysis, three candidate lipases exhibiting 97%, 55%, and 35% identities with CALA, respectively, were selected for overexpression and characterization. Lipase, PhLip from Pseudozyma hubeiensis SY62 showed highest activity towards long-chain fatty acids, and showed maximum activity at pH 9.0 and 60 °C, and stability between 40 and 50 °C for 4 h and at pH 7-10 for 12 h. Enzymatic hydrolysis of Mucor circinelloides WJ11 oils by PhLip was about twofold higher than that by CALA, with respect to hydrolysis of long-chain fatty acids. Besides, fatty acids with 18 carbons, including oleic acid, linoleic acid, and linolenic acid, were preferred as substrates.
CONCLUSION: The current investigation discovered a stable lipase PhLip with long-chain fatty acids preference. PhLip may be a potential candidate for producing polyunsaturated fatty acids from natural oils.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:42 |
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| Enthalten in: |
Biotechnology letters - 42(2020), 1 vom: 19. Jan., Seite 171-180 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Meiling [VerfasserIn] |
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| Links: |
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| Themen: |
CALA |
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| Anmerkungen: |
Date Completed 03.06.2020 Date Revised 03.06.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s10529-019-02765-4 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM303487615 |
|---|
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| 245 | 1 | 0 | |a Discovery and characterization of a stable lipase with preference toward long-chain fatty acids |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Completed 03.06.2020 | ||
| 500 | |a Date Revised 03.06.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVES: To identify novel lipases with stability and long-chain fatty acids preference by phylogenetic evolution analysis methods from database | ||
| 520 | |a RESULTS: Thermo-stable Candida antarctica Lipase-A (CALA) was set as a template for gene mining by PSI-BLAST. Based on phylogenetic analysis, three candidate lipases exhibiting 97%, 55%, and 35% identities with CALA, respectively, were selected for overexpression and characterization. Lipase, PhLip from Pseudozyma hubeiensis SY62 showed highest activity towards long-chain fatty acids, and showed maximum activity at pH 9.0 and 60 °C, and stability between 40 and 50 °C for 4 h and at pH 7-10 for 12 h. Enzymatic hydrolysis of Mucor circinelloides WJ11 oils by PhLip was about twofold higher than that by CALA, with respect to hydrolysis of long-chain fatty acids. Besides, fatty acids with 18 carbons, including oleic acid, linoleic acid, and linolenic acid, were preferred as substrates | ||
| 520 | |a CONCLUSION: The current investigation discovered a stable lipase PhLip with long-chain fatty acids preference. PhLip may be a potential candidate for producing polyunsaturated fatty acids from natural oils | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a CALA | |
| 650 | 4 | |a Enrichment | |
| 650 | 4 | |a Lipase | |
| 650 | 4 | |a Mucor circinelloides | |
| 650 | 4 | |a Phylogeny analysis | |
| 650 | 4 | |a Unsaturated fatty acid | |
| 650 | 7 | |a Fatty Acids |2 NLM | |
| 650 | 7 | |a Oils |2 NLM | |
| 650 | 7 | |a Lipase |2 NLM | |
| 650 | 7 | |a EC 3.1.1.3 |2 NLM | |
| 700 | 1 | |a Gao, Xiuzhen |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Wu |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Caili |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Junhuan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Huaiyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Yuanda |e verfasserin |4 aut | |
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