Anthocyanins from Hibiscus syriacus L. Inhibit Melanogenesis by Activating the ERK Signaling Pathway

Hibiscus syriacus L. exhibited promising potential as a new source of food and colorants containing various anthocyanins. However, the function of anthocyanins from H. syriacus L. has not been investigated. In the current study, we evaluated whether anthocyanins from the H. syriacus L. varieties Pulsae and Paektanshim (PS and PTS) inhibit melanin biogenesis. B16F10 cells and zebrafish larvae were exposed to PS and PTS in the presence or absence of α-melanocyte-stimulating hormone (α-MSH), and melanin contents accompanied by its regulating genes and proteins were analyzed. PS and PTS moderately downregulated mushroom tyrosinase activity in vitro, but significantly decreased extracellular and intracellular melanin production in B16F10 cells, and inhibited α-MSH-induced expression of microphthalmia-associated transcription factor (MITF) and tyrosinase. PS and PTS also attenuated pigmentation in α-MSH-stimulated zebrafish larvae. Furthermore, PS and PTS activated the phosphorylation of extracellular signal-regulated kinase (ERK), whereas PD98059, a specific ERK inhibitor, completely reversed PS- and PTS-mediated anti-melanogenic activity in B16F10 cells and zebrafish larvae, which indicates that PS- and PTS-mediated anti-melanogenic activity is due to ERK activation. Moreover, chromatography data showed that PS and PTS possessed 17 identical anthocyanins as a negative regulator of ERK. These findings suggested that anthocyanins from PS and PTS inhibited melanogenesis in vitro and in vivo by activating the ERK signaling pathway.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:9

Enthalten in:

Biomolecules - 9(2019), 11 vom: 24. Okt.

Sprache:

Englisch

Beteiligte Personen:

Karunarathne, Wisurumuni Arachchilage Hasitha Maduranga [VerfasserIn]
Molagoda, Ilandarage Menu Neelaka [VerfasserIn]
Park, Sang Rul [VerfasserIn]
Kim, Jeong Woon [VerfasserIn]
Lee, Oh-Kyu [VerfasserIn]
Kwon, Hae Yun [VerfasserIn]
Oren, Matan [VerfasserIn]
Choi, Yung Hyun [VerfasserIn]
Ryu, Hyung Won [VerfasserIn]
Oh, Sei-Ryang [VerfasserIn]
Jo, Wol Soon [VerfasserIn]
Lee, Kyoung Tae [VerfasserIn]
Kim, Gi-Young [VerfasserIn]

Links:

Volltext

Themen:

Anthocyanin
Anthocyanins
EC 2.7.11.24
ERK
Extracellular Signal-Regulated MAP Kinases
Hibiscus syriacus L.
Journal Article
Melanin
Melanins
Research Support, Non-U.S. Gov't
Tyrosinase

Anmerkungen:

Date Completed 22.09.2020

Date Revised 22.09.2020

published: Electronic

Citation Status MEDLINE

doi:

10.3390/biom9110645

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM302578382