CNDP2 Acts as an Activator for Human Ovarian Cancer Growth and Metastasis via the PI3K/AKT Pathway

INTRODUCTION: The mechanism of tumorigenesis and metastasis of ovarian cancer has not yet been elucidated. This study aimed to investigate the role and molecular mechanism of cytosolic nonspecific dipeptidase 2 in tumorigenesis and metastasis.

METHODS: Cytosolic nonspecific dipeptidase 2 expression in human ovarian cancer tissues and cell lines was assessed with methyl thiazolyl tetrazolium (MTT), clone formation, and transwell assays performed to evaluate the ability of ovarian cancer cells to proliferate and migrate. Nude mice tumor formation experiments were also performed by subcutaneously injecting cells with stable cytosolic nonspecific dipeptidase 2 knockdown and control SKOV3 cells into BALB/c female nude mice to detect changes in PI3K/AKT pathway-related proteins by Western blotting.

RESULTS: Cytosolic nonspecific dipeptidase 2 was highly expressed in human ovarian cancer tissues, with its expression associated with pathological data, including ovarian cancer metastasis. A cytosolic nonspecific dipeptidase 2 stable knockdown or ectopic expression ovarian cancer cell model was established and demonstrated that cytosolic nonspecific dipeptidase 2 could promote the proliferation of ovarian cancer cells. Transwell cell migration and invasion assays confirmed that cytosolic nonspecific dipeptidase 2 enhanced cell metastasis in ovarian cancer. Furthermore, in vivo xenograft experiments demonstrated that cytosolic nonspecific dipeptidase 2 can promote the development and progression of ovarian cancer, increasing the expression of phosphorylated PI3K and AKT.

CONCLUSIONS: Cytosolic nonspecific dipeptidase 2 promotes the occurrence and development of ovarian cancer through the PI3K/AKT signaling pathway.

Errataetall:

ExpressionOfConcernIn: Technol Cancer Res Treat. 2023 Jan-Dec;22:15330338231171228. - PMID 37163738

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:18

Enthalten in:

Technology in cancer research & treatment - 18(2019) vom: 01. Jan., Seite 1533033819874773

Sprache:

Englisch

Beteiligte Personen:

Zhang, Li Q [VerfasserIn]
Yang, Hua Q [VerfasserIn]
Yang, Su Q [VerfasserIn]
Wang, Ying [VerfasserIn]
Chen, Xian J [VerfasserIn]
Lu, Hong S [VerfasserIn]
Zhao, Ling P [VerfasserIn]

Links:

Volltext

Themen:

CNDP2
CNDP2 protein, human
Dipeptidases
EC 2.7.11.1
EC 3.4.13.-
EC 3.4.13.18
Growth
Journal Article
Metastasis
Ovarian cancer
Proto-Oncogene Proteins c-akt
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 26.02.2020

Date Revised 11.05.2023

published: Print

ExpressionOfConcernIn: Technol Cancer Res Treat. 2023 Jan-Dec;22:15330338231171228. - PMID 37163738

Citation Status MEDLINE

doi:

10.1177/1533033819874773

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM301452229