Cas9 regulated gene expression and pathogenicity in Riemerella anatipestifer
Copyright © 2019 Elsevier Ltd. All rights reserved..
Riemerellosis, a Riemerella anatipestifer infection, can cause meningitis, pericarditis, parahepatitis, and airsacculitis in ducks, leading to serious economic losses in the duck meat industry. However, the molecular mechanism of the pathogenesis and virulence factors of this infection are poorly understood. In the present study, we created a mutant strain RA-YMΔCas9 using trans-conjugation. Bacterial virulence tests indicated that the median lethal dose (LD50) of RA-YMΔCas9 was 5.01 × 107 CFU, significantly lower than that of the RA-YM strain, which was 1.58 × 105 CFU. The distribution and blood bacterial load from the infection groups showed no significant difference in the brain between the RA-YMΔCas9 mutant and the wild-type RA-YM strains, however, the number of mutant strains were significantly reduced in the liver, heart, and blood. Animal immunization experiments demonstrated that the intranasal administration of RA-YMΔCas9 in ducklings provided 80% protection after challenge with the wild-type strain, showing potential use as a live mucosal vaccine. RNAseq analysis indicated that Cas9 protein played a regulatory role in gene expression. This study is the first to report on the involvement of Cas9 in the regulation and pathogenesis of R. anatipestifer, and provides a theoretical basis for the development of relevant genetic engineering vaccines.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:136 |
|---|---|
| Enthalten in: |
Microbial pathogenesis - 136(2019) vom: 15. Nov., Seite 103706 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Ying [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
CRISPR-Associated Protein 9 |
|---|
| Anmerkungen: |
Date Completed 10.03.2020 Date Revised 10.03.2020 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.micpath.2019.103706 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM301004935 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM301004935 | ||
| 003 | DE-627 | ||
| 005 | 20231225103728.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.micpath.2019.103706 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1003.xml |
| 035 | |a (DE-627)NLM301004935 | ||
| 035 | |a (NLM)31491547 | ||
| 035 | |a (PII)S0882-4010(19)30170-6 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Ying |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Cas9 regulated gene expression and pathogenicity in Riemerella anatipestifer |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 10.03.2020 | ||
| 500 | |a Date Revised 10.03.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Riemerellosis, a Riemerella anatipestifer infection, can cause meningitis, pericarditis, parahepatitis, and airsacculitis in ducks, leading to serious economic losses in the duck meat industry. However, the molecular mechanism of the pathogenesis and virulence factors of this infection are poorly understood. In the present study, we created a mutant strain RA-YMΔCas9 using trans-conjugation. Bacterial virulence tests indicated that the median lethal dose (LD50) of RA-YMΔCas9 was 5.01 × 107 CFU, significantly lower than that of the RA-YM strain, which was 1.58 × 105 CFU. The distribution and blood bacterial load from the infection groups showed no significant difference in the brain between the RA-YMΔCas9 mutant and the wild-type RA-YM strains, however, the number of mutant strains were significantly reduced in the liver, heart, and blood. Animal immunization experiments demonstrated that the intranasal administration of RA-YMΔCas9 in ducklings provided 80% protection after challenge with the wild-type strain, showing potential use as a live mucosal vaccine. RNAseq analysis indicated that Cas9 protein played a regulatory role in gene expression. This study is the first to report on the involvement of Cas9 in the regulation and pathogenesis of R. anatipestifer, and provides a theoretical basis for the development of relevant genetic engineering vaccines | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cas9 | |
| 650 | 4 | |a Immunity | |
| 650 | 4 | |a RNAseq | |
| 650 | 4 | |a Riemerella anatipestifer | |
| 650 | 4 | |a Virulence | |
| 650 | 7 | |a Virulence Factors |2 NLM | |
| 650 | 7 | |a CRISPR-Associated Protein 9 |2 NLM | |
| 650 | 7 | |a EC 3.1.- |2 NLM | |
| 700 | 1 | |a Yin, Xuehuan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Zutao |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Sishun |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Shaowen |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiliang |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Yuncai |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Deshi |e verfasserin |4 aut | |
| 700 | 1 | |a Bi, Dingren |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zili |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Microbial pathogenesis |d 1987 |g 136(2019) vom: 15. Nov., Seite 103706 |w (DE-627)NLM012594156 |x 1096-1208 |7 nnns |
| 773 | 1 | 8 | |g volume:136 |g year:2019 |g day:15 |g month:11 |g pages:103706 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.micpath.2019.103706 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 136 |j 2019 |b 15 |c 11 |h 103706 | ||