A theory of centriole duplication based on self-organized spatial pattern formation
© 2019 Takao et al..
In each cell cycle, centrioles are duplicated to produce a single copy of each preexisting centriole. At the onset of centriole duplication, the master regulator Polo-like kinase 4 (Plk4) undergoes a dynamic change in its spatial pattern around the preexisting centriole, forming a single duplication site. However, the significance and mechanisms of this pattern transition remain unknown. Using super-resolution imaging, we found that centriolar Plk4 exhibits periodic discrete patterns resembling pearl necklaces, frequently with single prominent foci. Mathematical modeling and simulations incorporating the self-organization properties of Plk4 successfully generated the experimentally observed patterns. We therefore propose that the self-patterning of Plk4 is crucial for the regulation of centriole duplication. These results, defining the mechanisms of self-organized regulation, provide a fundamental principle for understanding centriole duplication.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:218 |
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| Enthalten in: |
The Journal of cell biology - 218(2019), 11 vom: 04. Nov., Seite 3537-3547 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Takao, Daisuke [VerfasserIn] |
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| Links: |
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| Themen: |
EC 2.7.1.- |
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| Anmerkungen: |
Date Completed 13.05.2020 Date Revised 04.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1083/jcb.201904156 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM300612273 |
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| 245 | 1 | 2 | |a A theory of centriole duplication based on self-organized spatial pattern formation |
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| 500 | |a Date Revised 04.12.2021 | ||
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2019 Takao et al. | ||
| 520 | |a In each cell cycle, centrioles are duplicated to produce a single copy of each preexisting centriole. At the onset of centriole duplication, the master regulator Polo-like kinase 4 (Plk4) undergoes a dynamic change in its spatial pattern around the preexisting centriole, forming a single duplication site. However, the significance and mechanisms of this pattern transition remain unknown. Using super-resolution imaging, we found that centriolar Plk4 exhibits periodic discrete patterns resembling pearl necklaces, frequently with single prominent foci. Mathematical modeling and simulations incorporating the self-organization properties of Plk4 successfully generated the experimentally observed patterns. We therefore propose that the self-patterning of Plk4 is crucial for the regulation of centriole duplication. These results, defining the mechanisms of self-organized regulation, provide a fundamental principle for understanding centriole duplication | ||
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| 700 | 1 | |a Kitagawa, Daiju |e verfasserin |4 aut | |
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