Destabilization of the Tumor-Inducing Plasmid from an Octopine-Type Agrobacterium tumefaciens Lineage Drives a Large Deletion in the Co-resident At Megaplasmid
Copyright © 2019 Barton et al..
Bacteria with multi-replicon genome organizations, including members of the family Rhizobiaceae, often carry a variety of niche-associated functions on large plasmids. While evidence exists for cross-replicon interactions and co-evolution between replicons in many of these systems, remarkable strain-to-strain variation is also observed for extrachromosomal elements, suggesting increased genetic plasticity. Here, we show that curing of the tumor-inducing virulence plasmid (pTi) of an octopine-type Agrobacterium tumefaciens lineage leads to a large deletion in the co-resident At megaplasmid (pAt). The deletion event is mediated by a repetitive IS-element, IS66, and results in a variety of environment-dependent fitness consequences, including loss of independent conjugal transfer of the plasmid. Interestingly, a related and otherwise wild-type A. tumefaciens strain is missing exactly the same large pAt segment as the pAt deletion derivatives, suggesting a similar event over its natural history. Overall, the findings presented here uncover a novel genetic interaction between the two large plasmids of A. tumefaciens and provide evidence for cross-replicon integration and co-evolution of these plasmids.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
G3 (Bethesda, Md.) - 9(2019), 10 vom: 07. Okt., Seite 3489-3500 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Barton, Ian S [VerfasserIn] |
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| Links: |
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| Themen: |
Accessory genome |
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| Anmerkungen: |
Date Completed 12.03.2020 Date Revised 12.03.2020 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1534/g3.119.400554 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM30061165X |
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| 100 | 1 | |a Barton, Ian S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Destabilization of the Tumor-Inducing Plasmid from an Octopine-Type Agrobacterium tumefaciens Lineage Drives a Large Deletion in the Co-resident At Megaplasmid |
| 264 | 1 | |c 2019 | |
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| 500 | |a Date Completed 12.03.2020 | ||
| 500 | |a Date Revised 12.03.2020 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019 Barton et al. | ||
| 520 | |a Bacteria with multi-replicon genome organizations, including members of the family Rhizobiaceae, often carry a variety of niche-associated functions on large plasmids. While evidence exists for cross-replicon interactions and co-evolution between replicons in many of these systems, remarkable strain-to-strain variation is also observed for extrachromosomal elements, suggesting increased genetic plasticity. Here, we show that curing of the tumor-inducing virulence plasmid (pTi) of an octopine-type Agrobacterium tumefaciens lineage leads to a large deletion in the co-resident At megaplasmid (pAt). The deletion event is mediated by a repetitive IS-element, IS66, and results in a variety of environment-dependent fitness consequences, including loss of independent conjugal transfer of the plasmid. Interestingly, a related and otherwise wild-type A. tumefaciens strain is missing exactly the same large pAt segment as the pAt deletion derivatives, suggesting a similar event over its natural history. Overall, the findings presented here uncover a novel genetic interaction between the two large plasmids of A. tumefaciens and provide evidence for cross-replicon integration and co-evolution of these plasmids | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a IS element | |
| 650 | 4 | |a Ti plasmid | |
| 650 | 4 | |a accessory genome | |
| 650 | 4 | |a conjugal transfer | |
| 650 | 4 | |a deletion | |
| 650 | 4 | |a plasmid curing | |
| 700 | 1 | |a Platt, Thomas G |e verfasserin |4 aut | |
| 700 | 1 | |a Rusch, Douglas B |e verfasserin |4 aut | |
| 700 | 1 | |a Fuqua, Clay |e verfasserin |4 aut | |
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