Kinetics of lipid raft formation at lipid monolayer-bilayer junction probed by surface plasmon resonance

Copyright © 2019 Elsevier B.V. All rights reserved..

A label-free, non-dispruptive, and real-time analytical device to monitor the dynamic features of biomolecules and their interactions with neighboring molecules is an essential prerequisite for biochip- and diagonostic assays. To explore one of the central questions on the lipid-lipid interactions in the course of the liquid-ordered (lo) domain formation, called rafts, we developed a method of reconstituting continuous but spatially heterogeneous lipid membrane platforms with molayer-bilayer juntions (MBJs) that enable to form the lo domains in a spatiotemporally controlled manner. This allows us to detect the time-lapse dynamics of the lipid-lipid interactions during raft formation and resultant membrane phase changes together with the raft-associated receptor-ligand binding through the surface plasmon resonance (SPR). For cross-validation, using epifluorescence microscopy, we demonstrated the underlying mechanisms for raft formations that the infiltration of cholesterols into the sphingolipid-enriched domains plays a crucial roles in the membrane phase-separation. Our membrane platform, being capable of monitoring dynamic interactions among lipids and performing the systematic optical analysis, will unveil physiological roles of cholesterols in a variety of biological events.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:142

Enthalten in:

Biosensors & bioelectronics - 142(2019) vom: 01. Okt., Seite 111568

Sprache:

Englisch

Beteiligte Personen:

Ryu, Yong-Sang [VerfasserIn]
Yun, Hansik [VerfasserIn]
Chung, Taerin [VerfasserIn]
Suh, Jeng-Hun [VerfasserIn]
Kim, Sungho [VerfasserIn]
Lee, Kyookeun [VerfasserIn]
Wittenberg, Nathan J [VerfasserIn]
Oh, Sang-Hyun [VerfasserIn]
Lee, Byoungho [VerfasserIn]
Lee, Sin-Doo [VerfasserIn]

Links:

Volltext

Themen:

97C5T2UQ7J
Cholesterol
Journal Article
Lipid Bilayers
Lipid monolayer-bilayer junction
Lipid raft
Lipid-lipid interaction
Optical sensors
Real-time
Surface plasmon resonance

Anmerkungen:

Date Completed 31.01.2020

Date Revised 31.01.2020

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.bios.2019.111568

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM300526687