Kinetics of lipid raft formation at lipid monolayer-bilayer junction probed by surface plasmon resonance
Copyright © 2019 Elsevier B.V. All rights reserved..
A label-free, non-dispruptive, and real-time analytical device to monitor the dynamic features of biomolecules and their interactions with neighboring molecules is an essential prerequisite for biochip- and diagonostic assays. To explore one of the central questions on the lipid-lipid interactions in the course of the liquid-ordered (lo) domain formation, called rafts, we developed a method of reconstituting continuous but spatially heterogeneous lipid membrane platforms with molayer-bilayer juntions (MBJs) that enable to form the lo domains in a spatiotemporally controlled manner. This allows us to detect the time-lapse dynamics of the lipid-lipid interactions during raft formation and resultant membrane phase changes together with the raft-associated receptor-ligand binding through the surface plasmon resonance (SPR). For cross-validation, using epifluorescence microscopy, we demonstrated the underlying mechanisms for raft formations that the infiltration of cholesterols into the sphingolipid-enriched domains plays a crucial roles in the membrane phase-separation. Our membrane platform, being capable of monitoring dynamic interactions among lipids and performing the systematic optical analysis, will unveil physiological roles of cholesterols in a variety of biological events.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2019 |
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Erschienen: |
2019 |
Enthalten in: |
Zur Gesamtaufnahme - volume:142 |
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Enthalten in: |
Biosensors & bioelectronics - 142(2019) vom: 01. Okt., Seite 111568 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Ryu, Yong-Sang [VerfasserIn] |
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Links: |
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Themen: |
97C5T2UQ7J |
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Anmerkungen: |
Date Completed 31.01.2020 Date Revised 31.01.2020 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.bios.2019.111568 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM300526687 |
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520 | |a Copyright © 2019 Elsevier B.V. All rights reserved. | ||
520 | |a A label-free, non-dispruptive, and real-time analytical device to monitor the dynamic features of biomolecules and their interactions with neighboring molecules is an essential prerequisite for biochip- and diagonostic assays. To explore one of the central questions on the lipid-lipid interactions in the course of the liquid-ordered (lo) domain formation, called rafts, we developed a method of reconstituting continuous but spatially heterogeneous lipid membrane platforms with molayer-bilayer juntions (MBJs) that enable to form the lo domains in a spatiotemporally controlled manner. This allows us to detect the time-lapse dynamics of the lipid-lipid interactions during raft formation and resultant membrane phase changes together with the raft-associated receptor-ligand binding through the surface plasmon resonance (SPR). For cross-validation, using epifluorescence microscopy, we demonstrated the underlying mechanisms for raft formations that the infiltration of cholesterols into the sphingolipid-enriched domains plays a crucial roles in the membrane phase-separation. Our membrane platform, being capable of monitoring dynamic interactions among lipids and performing the systematic optical analysis, will unveil physiological roles of cholesterols in a variety of biological events | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Lipid monolayer-bilayer junction | |
650 | 4 | |a Lipid raft | |
650 | 4 | |a Lipid-lipid interaction | |
650 | 4 | |a Optical sensors | |
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650 | 4 | |a Surface plasmon resonance | |
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650 | 7 | |a Cholesterol |2 NLM | |
650 | 7 | |a 97C5T2UQ7J |2 NLM | |
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700 | 1 | |a Chung, Taerin |e verfasserin |4 aut | |
700 | 1 | |a Suh, Jeng-Hun |e verfasserin |4 aut | |
700 | 1 | |a Kim, Sungho |e verfasserin |4 aut | |
700 | 1 | |a Lee, Kyookeun |e verfasserin |4 aut | |
700 | 1 | |a Wittenberg, Nathan J |e verfasserin |4 aut | |
700 | 1 | |a Oh, Sang-Hyun |e verfasserin |4 aut | |
700 | 1 | |a Lee, Byoungho |e verfasserin |4 aut | |
700 | 1 | |a Lee, Sin-Doo |e verfasserin |4 aut | |
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