Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Jet

Mycobacterial cell walls comprise thick and diverse lipids and glycolipids that act as a permeability barrier to antibiotics or other chemical agents. The use of OH radicals from a non-thermal plasma jet (NTPJ) for the inactivation of mycobacteria in aqueous solution was adopted as a novel approach. Addition of water vapor in a nitrogen plasma jet generated OH radicals, which converted to hydrogen peroxide (H2O2) that inactivated non-pathogenic Mycobacterium smegmatis and pathogenic Mycobacterium tuberculosis H37Rv. A stable plasma plume was obtained from a nitrogen plasma jet with 1.91 W of power, killing Escherichia coli and mycobacteria effectively, whereas addition of catalase decreased the effects of the former. Mycobacteria were more resistant than E. coli to NTPJ treatment. Plasma treatment enhanced intracellular ROS production and upregulation of genes related to ROS stress responses (thiolrelated oxidoreductases, such as SseA and DoxX, and ferric uptake regulator furA). Morphological changes of M. smegmatis and M. tuberculosis H37Rv were observed after 5 min treatment with N2+H2O plasma, but not of pre-incubated sample with catalase. This finding indicates that the bactericidal efficacy of NTPJ is related to the toxicity of OH and H2O2 radicals in cells. Therefore, our study suggests that NTPJ treatment may effectively control pulmonary infections caused by M. tuberculosis and nontuberculous mycobacteria (NTM) such as M. avium or M. abscessus in water.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:29

Enthalten in:

Journal of microbiology and biotechnology - 29(2019), 9 vom: 28. Sept., Seite 1401-1411

Sprache:

Englisch

Beteiligte Personen:

Lee, Chaebok [VerfasserIn]
Subhadra, Bindu [VerfasserIn]
Choi, Hei-Gwon [VerfasserIn]
Suh, Hyun-Woo [VerfasserIn]
Uhm, Han S [VerfasserIn]
Kim, Hwa-Jung [VerfasserIn]

Links:

Volltext

Themen:

059QF0KO0R
3352-57-6
Anti-Bacterial Agents
BBX060AN9V
Culture Media
Hydrogen Peroxide
Hydroxyl Radical
Hydroxyl radical
Journal Article
Mycobacteria
N762921K75
Nitrogen
Non-thermal plasma
Plasma Gases
ROS stress
Reactive Oxygen Species
Water

Anmerkungen:

Date Completed 15.01.2020

Date Revised 15.01.2020

published: Print

Citation Status MEDLINE

doi:

10.4014/jmb.1904.04060

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM300442041