Gonadotropin-Releasing Hormone in Regulation of Thymic Development in Rats : Profile of Thymic Cytokines

An increasing body of recent experimental data confirms the impact of neurohormones on fetal development and function of different body systems. The synthesis of many neurohormones starts in fetal tissues before the hypothalamic-pituitary-adrenal and hypothalamic-pituitary-gonadal systems are formed, and their high levels are detected in the bloodstream. Here, we studied the role of gonadotropin-releasing hormone (GnRH) in rat thymus development and tried to reveal possible mechanisms underlying the GnRH effects in early development. Western blotting and reverse transcription-polymerase chain reaction allowed us to identify receptor for GnRH in the fetal thymus with peak expression on embryonic days 17-18 (ED17-18). Blocking the receptors in utero on ED17 by a GnRH antagonist suppressed the concanavalin A-induced proliferative response of T cells in adults. GnRH (10-7 M) increased mRNA expression of interleukin (IL)-4, IL-10, IL-1β, interferon γ (IFNγ), and tumor necrosis factor α (TNFα) in the thymus of 18-day fetuses after an ex vivo culture for 24 h. The increased mRNA levels of the cytokines in the thymus were accompanied by increased numbers of CD4+ T helpers. Overall, the data obtained confirm the regulatory or morphogenetic effect of GnRH on fetal thymus development mediated by synthesis of thymic cytokines.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:20

Enthalten in:

International journal of molecular sciences - 20(2019), 16 vom: 19. Aug.

Sprache:

Englisch

Beteiligte Personen:

Melnikova, Victoria I [VerfasserIn]
Lifantseva, Nadezhda V [VerfasserIn]
Voronova, Svetlana N [VerfasserIn]
Zakharova, Liudmila A [VerfasserIn]

Links:

Volltext

Themen:

33515-09-2
Cytokines
GnRH receptor
Gonadotropin-Releasing Hormone
Gonadotropin-releasing hormone
Journal Article
Prenatal programming
Rat fetus
Receptors, LHRH
Thymic cytokines
Thymic development

Anmerkungen:

Date Completed 23.01.2020

Date Revised 25.02.2020

published: Electronic

Citation Status MEDLINE

doi:

10.3390/ijms20164033

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM300407629