Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved..
Biallelic inactivation of the retinoblastoma tumor suppressor gene (RB1) causes formation of retinoblastoma, a retinal eye tumor occurring in early childhood. Using the CRISPR/Cas9 nickase system, exon 1 of RB1 was deleted, including the RB1 promoter. As a result, sublines were generated carrying deletions of RB1 exon 1/promoter on one or both alleles.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2019 |
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Erschienen: |
2019 |
Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
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Enthalten in: |
Stem cell research - 39(2019) vom: 15. Aug., Seite 101517 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Menges, Julia [VerfasserIn] |
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Links: |
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Themen: |
EC 2.3.2.27 |
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Anmerkungen: |
Date Completed 27.04.2020 Date Revised 27.04.2020 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.scr.2019.101517 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM300152868 |
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520 | |a Biallelic inactivation of the retinoblastoma tumor suppressor gene (RB1) causes formation of retinoblastoma, a retinal eye tumor occurring in early childhood. Using the CRISPR/Cas9 nickase system, exon 1 of RB1 was deleted, including the RB1 promoter. As a result, sublines were generated carrying deletions of RB1 exon 1/promoter on one or both alleles | ||
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