Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved..

Biallelic inactivation of the retinoblastoma tumor suppressor gene (RB1) causes formation of retinoblastoma, a retinal eye tumor occurring in early childhood. Using the CRISPR/Cas9 nickase system, exon 1 of RB1 was deleted, including the RB1 promoter. As a result, sublines were generated carrying deletions of RB1 exon 1/promoter on one or both alleles.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:39

Enthalten in:

Stem cell research - 39(2019) vom: 15. Aug., Seite 101517

Sprache:

Englisch

Beteiligte Personen:

Menges, Julia [VerfasserIn]
Cremanns, Martina [VerfasserIn]
Steenpass, Laura [VerfasserIn]

Links:

Volltext

Themen:

EC 2.3.2.27
Journal Article
RB1 protein, human
Research Support, Non-U.S. Gov't
Retinoblastoma Binding Proteins
Ubiquitin-Protein Ligases

Anmerkungen:

Date Completed 27.04.2020

Date Revised 27.04.2020

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.scr.2019.101517

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM300152868